Assessment of Injection Volume Limits When Using On-Column Focusing with Microbore Liquid Chromatography

Mills, M.J., Maltas, J. and Lough, John (1997) Assessment of Injection Volume Limits When Using On-Column Focusing with Microbore Liquid Chromatography. Journal of Chromatography, 759 (1-2). pp. 1-11. ISSN S00219673

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Abstract

It had been ascertained that in the use of microbore liquid chromatography (LC) and on-column focusing (peak compression), there were advantages to be had in using the strongest eluting injection solvent that would still bring about on-column focusing. In such cases, it was found that the scope for the use of on-column focusing with microbore LC was greater than previously imagined. It has been possible to explain these results by considering on-column focusing to take place in two distinct phases, i.e. focusing of the sample band due to the injection solvent, followed by further focusing of the sample band by the sample solvent/mobile phase step gradient. In this content, an equation has been developed which provides a much more accurate estimate of the maximum allowable injection volume for a given injection solvent and mobile phase than has been possible until now.

Item Type: Article
Subjects: Sciences
Divisions: Faculty of Applied Sciences
Faculty of Applied Sciences > Department of Pharmacy Health and Wellbeing
Depositing User: Paula Normington
Date Deposited: 09 Jun 2017 11:01
Last Modified: 09 Jun 2017 11:01
URI: http://sure.sunderland.ac.uk/id/eprint/6416

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