Determination of cellular glutathioneglutathione disulfide ratio

Williams, Lee, Haroune, Nicolas, Childs, Stephen and Gronow, Michael (2016) Determination of cellular glutathioneglutathione disulfide ratio. Journal of Chromatography A (1437). pp. 67-73.

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Abstract

A validated method has been developed for the simultaneous measurement of reduced and oxidizedglutathione in de-proteinised cellular extracts. This has been used to compare models of malignant andnon-malignant human prostate cell lines. Analysis of LNCaP and DU145 cells showed a glutathione toglutathione disulfide ratio of 8:1 and 32:1 respectively, whilst the control cell line, PZ-HPV7 displayeda ratio of 93:1. Results indicate that the more aggressive phenotype displays adaptation to increasedoxidative stress via up regulation of glutathione turnover. It was also noted that in the LNCaP and DU145cell line, glutathione was only responsible for ca. 60% and 79% respectively, of the total cellular reducedthiol; indicating the presence of other biological thiols.

Item Type: Article
Subjects: Sciences > Chemistry
Divisions: Faculty of Applied Sciences > Department of Pharmacy Health and Wellbeing
Related URLs:
Depositing User: Lee Williams
Date Deposited: 31 Oct 2016 10:53
Last Modified: 20 Sep 2017 04:50
URI: http://sure.sunderland.ac.uk/id/eprint/6712

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