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The Chemical Characterization of Nigerian Propolis samples and Their Activity Against Trypanosoma brucei

Paget, Timothy (2017) The Chemical Characterization of Nigerian Propolis samples and Their Activity Against Trypanosoma brucei. Scientific reports, 7 (923). ISSN 2045-2322

Item Type: Article

Abstract

Profiling of extracts from twelve propolis samples collected from eight regions in Nigeria was carried out using high performance liquid chromatography (LC) coupled with evaporative light scattering (ELSD), ultraviolet detection (UV) and mass spectrometry (MS), gas chromatography mass spectrometry (GC-MS) and nuclear magnetic resonance spectroscopy (NMR). Principal component analysis (PCA) of the processed LC-MS data demonstrated the varying chemical composition of the samples. Most of the samples were active against Trypanosoma b.brucei with the highest activity being in the samples from Southern Nigeria. The more active samples were fractionated in order to isolate the component(s) responsible for their activity using medium pressure liquid chromatography (MPLC). Three xanthones, 1,3,7-trihydroxy-2,8-di-(3-methylbut-2-enyl)xanthone, 1,3,7-trihydroxy-4,8-di-(3-methylbut-2-enyl)xanthone a previously undescribed xanthone and three triterpenes: ambonic acid, mangiferonic acid and a mixture of α-amyrin with mangiferonic acid (1:3) were isolated and characterised by NMR and LC-MS. These compounds all displayed strong inhibitory activity against T.b.brucei but none of them had higher activity than the crude extracts. Partial least squares (PLS) modelling of the anti-trypanosomal activity of the sample extracts using the LC-MS data indicated that high activity in the extracts, as judged from LCMS
2data, could be correlated to denticulatain isomers in the extracts.

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Depositing User: Tim Paget

Identifiers

Item ID: 7178
Identification Number: https://doi.org/10.1038/s41598-017-01038-2
ISSN: 2045-2322
URI: http://sure.sunderland.ac.uk/id/eprint/7178
Official URL: https://www.nature.com/articles/s41598-017-01038-2

Users with ORCIDS

ORCID for Timothy Paget: ORCID iD orcid.org/0000-0002-9758-8044

Catalogue record

Date Deposited: 24 Apr 2017 15:07
Last Modified: 18 Dec 2019 15:40