Analysis of pollutant-induced changes in mitochondrial DNA methylation

Byun, Hyang-Min and Barrow, Timothy (2015) Analysis of pollutant-induced changes in mitochondrial DNA methylation. In: Mitochondrial Medicine: Volume 2 - Manipulating Mitochondrial Function. Methods in Molecular Biology . Humana Press, pp. 271-283. ISBN 978-1-4939-2288-8

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Abstract

There is increasing evidence that exposure to air pollutants are associated with human disease and may act through epigenetic modification of the nuclear genome, but there have been few publications describing their impact upon the mitochondrial genome. Mitochondrial DNA may be more susceptible to pollutant-induced changes via increased oxidative stress in the cell, and therefore this field of research is of growing interest. Many techniques employed to study DNA methylation of the nuclear genome are also applicable to mitochondrial epigenetic studies. In this chapter, we describe a protocol for the isolation of mitochondrial DNA from peripheral blood samples and the analysis of 5-methlycytosine content by bisulfite-pyrosequencing.

Item Type: Book Section
Subjects: Sciences > Biomedical Sciences
Divisions: Faculty of Health Sciences and Wellbeing
Depositing User: Timothy Barrow
Date Deposited: 26 Jul 2018 15:16
Last Modified: 26 Jul 2018 15:16
URI: http://sure.sunderland.ac.uk/id/eprint/9740

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