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Stimulation of erythrocyte ceramide formation by platelet-activating factor.

Lang, Philipp A, Kempe, Daniela S, Tanneur, Valerie, Eisele, Kerstin, Klarl, Barbara A, Mysina, Svetlana, Jendrossek, Verena, Ishii, Satoshi, Shimizu, Takao, Waidmann, Marc, Hessler, Gabriele, Huber, Stephan M, Lang, Florian and Wieder, Thomas (2005) Stimulation of erythrocyte ceramide formation by platelet-activating factor. Journal of cell science, 118 (Pt 6). pp. 1233-43. ISSN 0021-9533

Item Type: Article

Abstract

Osmotic erythrocyte shrinkage leads to activation of cation channels with subsequent Ca2+ entry and stimulates a sphingomyelinase with subsequent formation of ceramide. Ca2+ and ceramide then activate a scramblase leading to breakdown of phosphatidylserine asymmetry of the cell membrane. The mediators accounting for activation of erythrocyte sphingomyelinase and phosphatidylserine exposure remained elusive. The study demonstrates that platelet-activating factor (PAF) is released from erythrocytes upon hyperosmotic cell shrinkage. The experiments further disclose the presence of PAF receptors in erythrocytes and show that PAF stimulates the breakdown of sphingomyelin and the release of ceramide from erythrocytes at isotonic conditions. PAF further triggers cell shrinkage (decrease of forward scatter) and phosphatidylserine exposure (annexin binding) of erythrocytes. The stimulation of annexin-binding is blunted by a genetic knockout of PAF receptors, by the PAF receptor antagonist ABT491 or by inhibition of sphingomyelinase with urea. In conclusion, PAF activates an erythrocyte sphingomyelinase and the then formed ceramide leads to the activation of scramblase with subsequent phosphatidylserine exposure.

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More Information

Depositing User: Svetlana Mysina

Identifiers

Item ID: 10021
ISSN: 0021-9533
URI: http://sure.sunderland.ac.uk/id/eprint/10021

Users with ORCIDS

Catalogue record

Date Deposited: 08 Oct 2018 09:17
Last Modified: 09 Oct 2018 10:38

Contributors

Author: Philipp A Lang
Author: Daniela S Kempe
Author: Valerie Tanneur
Author: Kerstin Eisele
Author: Barbara A Klarl
Author: Svetlana Mysina
Author: Verena Jendrossek
Author: Satoshi Ishii
Author: Takao Shimizu
Author: Marc Waidmann
Author: Gabriele Hessler
Author: Stephan M Huber
Author: Florian Lang
Author: Thomas Wieder

Subjects

Sciences > Biomedical Sciences

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