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Differential expression of immunoregulatory genes in monocytes in response to Porphyromonas gingivalis and Escherichia coli lipopolysaccharide.

Barksby, Emma, Nile, Chris, Jaedicke, Katrin Monika, Taylor, John and Preshaw, Philip (2009) Differential expression of immunoregulatory genes in monocytes in response to Porphyromonas gingivalis and Escherichia coli lipopolysaccharide. Clinical & Experimental Immunology, 156 (3). pp. 479-487. ISSN 0009-9104

Item Type: Article

Abstract

Porphyromonas gingivalis lipopolysaccharide (LPS) (strain W50) interacts with Toll-like receptor 2 (TLR-2) leading to cytokine expression and inflammation, and thereby plays a key role in the pathogenesis of periodontal disease. The aims of this study were to investigate gene expression of key regulatory mediators of innate immune responses in a human monocytic cell line (THP-1) to P. gingivalis LPS and to compare these results with those obtained using the TLR-4 ligand, Escherichia coli LPS. Custom-made Taqman low-density arrays were used for expression profiling of 45 different cytokine-related genes. Both types of LPS highly up-regulated interleukin (IL)-1alpha and IL-1beta, IL-18 receptor (IL-18R), IL-18R accessory protein and IL-1 family (IL-1F)9. Expression levels of IL-1F6, IL-1F7 and caspase-1 were unaltered by either LPS. Genes for tumour necrosis factor-alpha, IL-6, leukaemia inhibitory factor and IL-32 were also highly induced by both LPS. For a subset of genes, including CXC chemokine ligand 5 (CXCL5), expression was induced only by E. coli LPS or was up-regulated more highly by E. coli compared with P. gingivalis LPS in THP-1 monocytes. A similar expression pattern was also observed in dendritic cells. Analysis of signalling pathways which lead to CXCL5 expression indicated that the mechanisms underpinning the differential responses did not involve the recruitment of different adaptor proteins by TLR-2 and TLR-4, and therefore occur downstream of the receptor-adaptor complex. We conclude that differences in signalling pathways activated by TLR-2 and TLR-4 ligands lead to differential innate immune responses which may be important in polymicrobial diseases such as periodontal disease.

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More Information

Depositing User: Katrin Jaedicke

Identifiers

Item ID: 9594
Identification Number: https://doi.org/10.1111/j.1365-2249.2009.03920.x
ISSN: 0009-9104
URI: http://sure.sunderland.ac.uk/id/eprint/9594
Official URL: https://onlinelibrary.wiley.com/doi/abs/10.1111/j....

Users with ORCIDS

ORCID for Katrin Monika Jaedicke: ORCID iD orcid.org/0000-0002-7389-5816

Catalogue record

Date Deposited: 31 May 2018 14:53
Last Modified: 20 May 2019 12:15

Contributors

Author: Katrin Monika Jaedicke ORCID iD
Author: Emma Barksby
Author: Chris Nile
Author: John Taylor
Author: Philip Preshaw

University Divisions

Faculty of Health Sciences and Wellbeing

Subjects

Sciences > Biomedical Sciences
Sciences

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