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Nano-encapsulation of a novel anti-Ran-GTPase peptide for blockade of regulator of chromosome condensation 1 (RCC1) function in MDA-MB-231 breast cancer cells

Haggag, Yusuf. A, Matchett, Kyle B., Dakirc, El-Habib, Buchanan, Paul, Osman, Mohammed A., Elgizawyb, Sanaa A., El-Tanani, Mohamed, Faheem, Ahmed and McCarron, Paul A. (2017) Nano-encapsulation of a novel anti-Ran-GTPase peptide for blockade of regulator of chromosome condensation 1 (RCC1) function in MDA-MB-231 breast cancer cells. International Journal of Pharmaceutics, 521 (1-2). pp. 40-53. ISSN 0378-5173

Item Type: Article

Abstract

Ran is a small ras-related GTPase and is highly expressed in aggressive breast carcinoma. Overexpression induces malignant transformation and drives metastatic growth. We have designed a novel series of anti-Ran-GTPase peptides, which prevents Ran hydrolysis and activation, and although they display effectiveness in silico, peptide activity is suboptimal in vitro due to reduced bioavailability and poor delivery. To overcome this drawback, we delivered an anti-Ran-GTPase peptide using encapsulation in PLGA-based nanoparticles (NP). Formulation variables within a double emulsion solvent evaporation technique were controlled to optimise physicochemical properties. NP were spherical and negatively charged with a mean diameter of 182–277 nm. Peptide integrity and stability were maintained after encapsulation and release kinetics followed a sustained profile. We were interested in the relationship between cellular uptake and poly(ethylene glycol) (PEG) in the NP matrix, with results showing enhanced in vitro uptake with increasing PEG content. Peptide-loaded, pegylated (10% PEG)-PLGA NP induced significant cytotoxic and apoptotic effects in MDA-MB-231 breast cancer cells, with no evidence of similar effects in cells pulsed with free peptide. Western blot analysis showed that encapsulated peptide interfered with the proposed signal transduction pathway of the Ran gene. Our novel blockade peptide prevented Ran activation by blockage of regulator of chromosome condensation 1 (RCC1) following peptide release directly in the cytoplasm once endocytosis of the peptide-loaded nanoparticle has occurred. RCC1 blockage was effective only when a nanoparticulate delivery approach was adopted.

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Depositing User: Callum Williams

Identifiers

Item ID: 7191
Identification Number: https://doi.org/10.1016/j.ijpharm.2017.02.006
ISSN: 0378-5173
URI: http://sure.sunderland.ac.uk/id/eprint/7191
Official URL: http://doi.org/10.1016/j.ijpharm.2017.02.006

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Catalogue record

Date Deposited: 28 Apr 2017 09:14
Last Modified: 18 Dec 2019 15:40

Contributors

Author: Yusuf. A Haggag
Author: Kyle B. Matchett
Author: El-Habib Dakirc
Author: Paul Buchanan
Author: Mohammed A. Osman
Author: Sanaa A. Elgizawyb
Author: Mohamed El-Tanani
Author: Ahmed Faheem
Author: Paul A. McCarron

University Divisions

Faculty of Health Sciences and Wellbeing
Faculty of Health Sciences and Wellbeing > School of Pharmacy and Pharmaceutical Sciences

Subjects

Sciences > Pharmacy and Pharmacology

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