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Effect of processed and red meat on endogenous nitrosation and DNA damage

Joosen, Annemiek, Kuhnle, Gunter, Aspinall, Sue, Barrow, Timothy, Lecommandeur, Emmanuelle, Azqueta, Amaya, Collins, Andrew and Bingham, Sheila (2009) Effect of processed and red meat on endogenous nitrosation and DNA damage. Carcinogenesis, 30 (8). pp. 1402-1407. ISSN 0143-3334

Item Type: Article

Abstract

Haem in red meat (RM) stimulates the endogenous production of mutagenic nitroso compounds (NOC). Processed (nitrite-preserved red) meat additionally contains high concentrations of preformed NOC. In two studies, of a fresh RM versus a vegetarian (VEG) diet (six males and six females) and of a nitrite-preserved red meat (PM) versus a VEG diet (5 males and 11 females), we investigated whether processing of meat might increase colorectal cancer risk by stimulating nitrosation and DNA damage. Meat diets contained 420 g (males) or 366 g (females) meat/per day. Faecal homogenates from day 10 onwards were analysed for haem and NOC and associated supernatants for genotoxicity. Means are adjusted for differences in male to female ratios between studies. Faecal NOC concentrations on VEG diets were low (2.6 and 3.5 mmol/g) but significantly higher on meat diets (PM 175 +/- 19 nmol/g versus RM 185 +/- 22 nmol/g; P = 0.75). The RM diet resulted in a larger proportion of nitrosyl iron (RM 78% versus PM 54%; P < 0.0001) and less nitrosothiols (RM 12% versus PM 19%; P < 0.01) and other NOC (RM 10% versus PM 27%; P < 0.0001). There was no statistically significant difference in DNA breaks induced by faecal water (FW) following PM and RM diets (P = 0.80). However, PM resulted in higher levels of oxidized pyrimidines (P < 0.05). Surprisingly, VEG diets resulted in significantly more FW-induced DNA strand breaks than the meat diets (P < 0.05), which needs to be clarified in further studies. Meats cured with nitrite have the same effect as fresh RM on endogenous nitrosation but show increased FW-induced oxidative DNA damage.

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Depositing User: Timothy Barrow

Identifiers

Item ID: 9737
Identification Number: https://doi.org/10.1093/carcin/bgp130
ISSN: 0143-3334
URI: http://sure.sunderland.ac.uk/id/eprint/9737
Official URL: https://academic.oup.com/carcin/article/30/8/1402/...

Users with ORCIDS

ORCID for Timothy Barrow: ORCID iD orcid.org/0000-0003-4551-3857

Catalogue record

Date Deposited: 13 Jul 2018 10:37
Last Modified: 18 Dec 2019 16:07

Contributors

Author: Timothy Barrow ORCID iD
Author: Annemiek Joosen
Author: Gunter Kuhnle
Author: Sue Aspinall
Author: Emmanuelle Lecommandeur
Author: Amaya Azqueta
Author: Andrew Collins
Author: Sheila Bingham

University Divisions

Faculty of Health Sciences and Wellbeing
Faculty of Health Sciences and Wellbeing > School of Nursing and Health Sciences

Subjects

Sciences > Biomedical Sciences

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