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Comparison of Mycobacterial Growth Indicator Tube with Culture on RGM Selective Agar for Detection of Mycobacteria in Sputum Samples from Patients with Cystic Fibrosis

Eltringham, Ian, Pickering, Julie, Gough, Helen, Preece, Clair L., Perry, John D. and Forbes, B. A. (2016) Comparison of Mycobacterial Growth Indicator Tube with Culture on RGM Selective Agar for Detection of Mycobacteria in Sputum Samples from Patients with Cystic Fibrosis. Journal of Clinical Microbiology, 54 (8). pp. 2047-2050. ISSN 0095-1137

Item Type: Article

Abstract

Nontuberculous mycobacteria (NTM) are an important cause of pulmonary disease in patients with cystic fibrosis (CF). A new culture medium (RGM medium) for the isolation of rapidly growing mycobacteria from the sputum of cystic fibrosis patients has recently been reported. The aim of this study was to compare culture of sputum samples on RGM medium with culture using a standard automated liquid culture method. Sputum samples were obtained from 187 distinct patients with CF attending King's College Hospital, London, United Kingdom. Each sample was decontaminated with 3% oxalic acid and inoculated into a mycobacterial growth indicator tube (MGIT) that was monitored for 42 days using the Bactec MGIT 960 instrument. Each sample was also cultured, without decontamination, onto RGM medium, which was incubated for 10 days at 30°C. Mycobacteria were isolated from 28 patients (prevalence, 15%). Mycobacteria were detected in 24 samples (86%) using the MGIT and in 23 samples (82%) using RGM medium (P = 1.00). In this setting, RGM medium showed sensitivity equivalent to that of the MGIT for isolation of NTM from the sputum of patients with CF. RGM medium offers a simple, convenient tool that can be embedded into routine culture methods, allowing the culture of all sputum samples that are submitted from patients with CF.

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More Information

Depositing User: Clair Preece

Identifiers

Item ID: 15915
Identification Number: https://doi.org/10.1128/JCM.00630-16
ISSN: 0095-1137
URI: http://sure.sunderland.ac.uk/id/eprint/15915
Official URL: http://dx.doi.org/10.1128/JCM.00630-16

Users with ORCIDS

ORCID for Clair L. Preece: ORCID iD orcid.org/0000-0002-1290-0423

Catalogue record

Date Deposited: 27 Apr 2023 13:45
Last Modified: 27 Apr 2023 13:45

Contributors

Author: Clair L. Preece ORCID iD
Author: Ian Eltringham
Author: Julie Pickering
Author: Helen Gough
Author: John D. Perry
Author: B. A. Forbes

University Divisions

Faculty of Health Sciences and Wellbeing

Subjects

Sciences > Biomedical Sciences
Sciences > Health Sciences

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