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Unconventional T-cell recognition of an arthritogenic epitope of proteoglycan aggrecan released from degrading cartilage

Falconer, Jane, Mahida, R, Venkatesh, D, Pearson, J and Robinson, JH (2016) Unconventional T-cell recognition of an arthritogenic epitope of proteoglycan aggrecan released from degrading cartilage. Immunology, 147 (4). pp. 389-398. ISSN 1365-2567

Item Type: Article

Abstract

It has been proposed that peptide epitopes bind to MHC class II molecules to form distinct structural conformers of the same MHC II–peptide complex termed type A and type B, and that the two conformers of the same peptide–MHC II complex are recognized by distinct CD4 T cells, termed type A and type B T cells. Both types recognize short synthetic peptides but only type A recognize endosomally processed intact antigen. Type B T cells that recognize self peptides from exogenously degraded proteins have been shown to escape negative selection during thymic development and so have the potential to contribute to the pathogenesis of autoimmunity. We generated and characterized mouse CD4 T cells specific for an arthritogenic epitope of the candidate joint autoantigen proteoglycan aggrecan. Cloned T-cell hybridomas specific for a synthetic peptide containing the aggrecan epitope showed two distinct response patterns based on whether they could recognize processed intact aggrecan. Fine mapping demonstrated that both types of T-cell recognized the same core epitope. The results are consistent with the generation of aggrecan-specific type A and type B T cells. Type B T cells were activated by supernatants released from degrading cartilage, indicating the presence of antigenic extracellular peptides or fragments of aggrecan. Type B T cells could play a role in the pathogenesis of proteoglycan-induced arthritis in mice, a model for rheumatoid arthritis, by recognizing extracellular peptides or protein fragments of joint autoantigens released by inflamed cartilage.

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More Information

Depositing User: Jane Falconer

Identifiers

Item ID: 11086
Identification Number: https://doi.org/10.1111/imm.12557
ISSN: 1365-2567
URI: http://sure.sunderland.ac.uk/id/eprint/11086
Official URL: https://onlinelibrary.wiley.com/doi/full/10.1111/i...

Users with ORCIDS

ORCID for Jane Falconer: ORCID iD orcid.org/0000-0002-6601-7919

Catalogue record

Date Deposited: 05 Sep 2019 12:15
Last Modified: 30 Sep 2020 11:30

Contributors

Author: Jane Falconer ORCID iD
Author: R Mahida
Author: D Venkatesh
Author: J Pearson
Author: JH Robinson

University Divisions

Faculty of Health Sciences and Wellbeing > School of Medicine

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