Close menu


Sunderland Repository records the research produced by the University of Sunderland including practice-based research and theses.

Chromatography: Separation Techniques in Biology

Lough, John and Carlile, Mark (2019) Chromatography: Separation Techniques in Biology. In: Biomolecular and Bioanalytical Techniques. Wiley, pp. 123-152. ISBN 9781119483960

Item Type: Book Section


Chromatography is a widely used analytical technique because of its resolving power, being able to separate sample components from one another prior to measurement. Nonetheless samples encountered in biology present a formidable challenge. They are often very complex, dynamic in nature, contain a diverse set of difficult‐to‐analyse compounds and may require the determination of analytes that are present at very low levels. Proteins, for example, are very large molecules with inherent slow mass transfer that may denature during analysis. While reversed‐phase chromatography may be applicable for the majority of small molecule separations, affinity chromatography, size exclusion chromatography, hydrophobic interaction chromatography and ion exchange chromatography might all need to be investigated before arriving at a suitable analytical method for proteins. In general, the complexity of biological samples is such that mass spectrometric detection in chromatographic methods is now well established and multidimensional chromatographic techniques are increasingly being used for very complex mixtures of small biological molecules such as protein digests.

Full text not available from this repository.

More Information

Depositing User: Leah Maughan


Item ID: 11536
Identification Number:
ISBN: 9781119483960
Official URL:

Users with ORCIDS

Catalogue record

Date Deposited: 13 Feb 2020 10:06
Last Modified: 13 Feb 2020 10:06


Author: John Lough
Author: Mark Carlile

University Divisions

Faculty of Health Sciences and Wellbeing > School of Pharmacy and Pharmaceutical Sciences

Actions (login required)

View Item View Item