Real-time cell cycle imaging during melanoma growth, invasion, and drug response

Haass, Nikolas K., Beaumont, Kimberley A., Hill, David, Anfosso, Andrea, Mrass, Paulus, Munoz, Marcia A., Kinjyo, Ichiko and Weninger, Wolfgang (2014) Real-time cell cycle imaging during melanoma growth, invasion, and drug response. Pigment Cell & Melanoma Research, 27 (5). pp. 764-776. ISSN 1755-1471

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Abstract

Solid cancers are composed of heterogeneous zones containing proliferating and quiescent cells. Despite considerable insight into the molecular mechanisms underlying aberrant cell cycle progression, there is limited understanding of the relationship between the cell cycle on the one side, and melanoma cell motility, invasion, and drug sensitivity on the other side. Utilizing the fluorescent ubiquitination‐based cell cycle indicator (FUCCI) to longitudinally monitor proliferation and migration of melanoma cells in 3D culture and in vivo, we found that invading melanoma cells cycle actively, while G1‐arrested cells showed decreased invasion. Melanoma cells in a hypoxic environment or treated with mitogen‐activated protein kinase pathway inhibitors remained G1‐arrested for extended periods of time, with proliferation and invasion resuming after re‐exposure to a more favorable environment. We challenge the idea that the invasive and proliferative capacity of melanoma cells are mutually exclusive and further demonstrate that a reversibly G1‐arrested subpopulation survives in the presence of targeted therapies.

Item Type: Article
Divisions: Faculty of Health Sciences and Wellbeing > School of Nursing and Health Sciences
Depositing User: Leah Maughan
Date Deposited: 06 Nov 2020 15:47
Last Modified: 06 Nov 2020 15:47
URI: http://sure.sunderland.ac.uk/id/eprint/12770
ORCID for David Hill: ORCID iD orcid.org/0000-0002-6080-0603

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