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PEGylated polymeric nanocapsules for oral delivery of trypsin targeted to the small intestines

Abu Abed, Omar S, Chaw, Cheng, Williams, Lee and Elkordy, Amal (2021) PEGylated polymeric nanocapsules for oral delivery of trypsin targeted to the small intestines. International Journal of Pharmaceutics, 592 (120094). ISSN 0378-5173

Item Type: Article


The lack of trypsin in the intestines may end up with malnutrition; thus, trypsin replacement therapy is required in such cases. The main objective of this study is to formulate and evaluate polymeric nanocapsule (PNC) systems able to deliver trypsin to the small intestines with the minimal release in the stomach with the maximum biological activity. Four nanocapsule formulations were prepared by double emulsion/evaporation method as w/o/w and s/o/w. Particle size, encapsulation efficiencies, drug release in simulated gastric fluids (SGF) and simulated intestinal fluids (SIF), morphology, the biological activity of encapsulated trypsin and shelf-life stability were investigated for all formulations. All formulations had a spherical shape with submicron size, and encapsulation efficiency more than 80%. The biological activity of encapsulated trypsin was significantly affected by the amount of trehalose and whether the formulations were prepared as s/o/w or w/o/w (P < 0.05). Most of the encapsulated protein was released sustainedly at the target site (SIF) over 24 h with minimum amount release in the gastric fluids. Also, more than 90% of physical integrity trypsin encapsulated in all formulations was retained after storage under chilled conditions for six months. However, the enzymatic assay results show that with low trehalose content, the biological activity was low, while increasing the trehalose amount increased the shelf stability to reach around 100% after six months of the study. The results obtained in this research work clearly indicated a promising potential of controlled release polymeric nanocapsules containing trypsin to target the small intestine and protect trypsin from the harsh condition facing the proteins during the process of preparation or the period of storage.

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Depositing User: Leah Maughan


Item ID: 12798
Identification Number:
ISSN: 0378-5173
Official URL:

Users with ORCIDS

ORCID for Cheng Chaw: ORCID iD
ORCID for Amal Elkordy: ORCID iD

Catalogue record

Date Deposited: 17 Nov 2020 16:26
Last Modified: 10 Nov 2021 03:38


Author: Cheng Chaw ORCID iD
Author: Amal Elkordy ORCID iD
Author: Omar S Abu Abed
Author: Lee Williams

University Divisions

Faculty of Health Sciences and Wellbeing > School of Pharmacy and Pharmaceutical Sciences

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