Novel oral pegylated polymeric nanoparticle for the delivery of trypsin targeted to the small intestine

Abu Abed, Omar S, Chaw, Cheng, Williams, Lee and Elkordy, Amal (2020) Novel oral pegylated polymeric nanoparticle for the delivery of trypsin targeted to the small intestine. International Journal of Pharmaceutics. ISSN 0378-5173

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The lack of trypsin in the intestines may end up with malnutrition; thus, trypsin replacement therapy is required in such cases. The objective of this study was to formulate and evaluate polymeric nanocapsule (PNC) systems able to deliver trypsin to the small intestine with the minimal release in the stomach with the maximum biological activity. Four nanocapsule formulations were prepared by double emulsion/evaporation method as w/o/w and s/o/w. Particle size, encapsulation efficiencies, drug release in simulated gastric fluids (SGF) and simulated intestinal fluids (SIF), morphology, the biological activity of encapsulated trypsin and shelf-life stability were investigated for all formulations. All formulations had a spherical shape with submicron size, and encapsulation efficiency more than 80%. The biological activity of encapsulated trypsin was significantly affected by the amount of trehalose and whether the formulations were prepared as s/o/w or w/o/w (P<0.05). Most of the encapsulated protein was sustained released at the target site (SIF) over 24 hours with minimum amount release in the gastric fluids. Also, more than 90% of physical integrity trypsin encapsulated in all formulations was retained after storage under chilled conditions for six months. However, the enzymatic assay results show that with low trehalose content, the biological activity was low, while increasing the trehalose amount increased the shelf stability to reach around 100% after six months of the study. The results obtained in this research work clearly indicated a promising potential of controlled release polymeric nanocapsules containing trypsin to target the small intestine and protect trypsin from the harsh condition facing the proteins during the process of preparation or the period of storage.

Item Type: Article
Divisions: Faculty of Health Sciences and Wellbeing > School of Pharmacy and Pharmaceutical Sciences
Depositing User: Leah Maughan
Date Deposited: 17 Nov 2020 16:26
Last Modified: 17 Nov 2020 16:30
ORCID for Cheng Chaw: ORCID iD
ORCID for Amal Elkordy: ORCID iD

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