Cystic ovary disease impairs transport speed, smooth muscle contraction, and epithelial ion transport in the bovine oviduct

Scully, Deirdre M., Campion, Deirdre, McCartney, Fiona, Dulohery, Kate, Reese, Sven and Kölle, Sabine (2021) Cystic ovary disease impairs transport speed, smooth muscle contraction, and epithelial ion transport in the bovine oviduct. Molecular Reproduction and Development. ISSN 1098-2795

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Abstract

Abstract: Cystic ovary disease (COD) is a common cause of bovine infertility but the impact of this disease on the oviduct is unknown. The aim of this study was to analyze the effects of COD on particle transport speed (PTS), ciliary beat frequency, myosalpinx contraction, and epithelial ion transport. Oviducts were obtained from cows affected by COD and compared with those of healthy, mid‐diestrus cows. PTS and CBF were examined using live‐cell imaging. Smooth muscle contraction and epithelial ion transport were investigated using organ baths and Ussing chambers. Our results showed that muscarinic receptors are involved in cholinergic signaling in the oviduct and that forskolin‐induced cyclic AMP production is involved in active ion transport in the oviductal epithelium. Oviducts from cows with luteal cysts revealed significantly decreased PTS (p = 0.02). Further to that, in the oviducts of COD cows, the cholinergic regulation of smooth muscle contractions and active epithelial ion transport were significantly diminished (p < 0.0001). These results imply that in COD cows, oviductal transport is compromised by decreased fluid flow speed and reduced cholinergic regulation of smooth muscle contraction and ion transport. This knowledge contributes to a more comprehensive understanding of COD supporting the development of novel therapeutic concepts for infertility treatment.

Item Type: Article
Additional Information: ** Article version: VoR ** From Wiley via Jisc Publications Router ** History: received 10-02-2021; rev-recd 28-04-2021; accepted 16-06-2021; pub-electronic 23-06-2021. ** Licence for VoR version of this article: http://creativecommons.org/licenses/by-nc-nd/4.0/
Uncontrolled Keywords: RESEARCH ARTICLE, RESEARCH ARTICLES, bovine oviduct, cholinergic regulation, cystic ovary disease, particle transport speed, smooth muscle contraction
Divisions: Faculty of Health Sciences and Wellbeing > School of Medicine
Related URLs:
SWORD Depositor: Publication Router
Depositing User: Publication Router
Date Deposited: 13 Jul 2021 15:06
Last Modified: 13 Jul 2021 15:06
URI: http://sure.sunderland.ac.uk/id/eprint/13630
ORCID for Deirdre M. Scully: ORCID iD orcid.org/0000-0002-5918-5615
ORCID for Deirdre Campion: ORCID iD orcid.org/0000-0001-5713-8973
ORCID for Fiona McCartney: ORCID iD orcid.org/0000-0002-5834-7950
ORCID for Kate Dulohery: ORCID iD orcid.org/0000-0003-4312-1366
ORCID for Sven Reese: ORCID iD orcid.org/0000-0002-4605-9791
ORCID for Sabine Kölle: ORCID iD orcid.org/0000-0002-4541-0498

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