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Sunderland Repository records the research produced by the University of Sunderland including practice-based research and theses.

Differential regulation of naïve and memory CD4+ T cells by alternatively activated dendritic cells.

Anderson, Amy E, Sayers, Bethan L, Haniffa, Muzlifah A, Swan, David, Diboll, Julie, Wang, Xiao-Nong, Isaacs, John D and Hilkens, Catharien M U (2008) Differential regulation of naïve and memory CD4+ T cells by alternatively activated dendritic cells. Journal of leukocyte biology, 84 (1). pp. 124-133. ISSN 0741-5400

Item Type: Article


Promising immunotherapeutic tools for T cell-mediated pathologies are alternatively activated dendritic cells (aaDC), which exert their effect through the regulation and tolerization of T cells. As naïve and memory T cells have different susceptibilities to tolerogenic signals, it is important to understand the modulatory effects of aaDC on these T cell subsets. We have examined regulation of naïve and memory CD4+ T cells by human aaDC generated with dexamethasone, the active form of vitamin D3, 1alpha,25-dihydroxyvitamin D3, and LPS. Although aaDC induced low, primary, allogeneic responses by naïve and memory T cells, aaDC regulated the differentiation of these T cell subsets in a distinct manner. Naïve T cells primed by aaDC retained a strong, proliferative capacity upon restimulation but were skewed toward a low IFN-gamma/high IL-10 cytokine profile. In contrast, memory T cells primed by aaDC became hyporesponsive in terms of proliferation and cytokine production. Induction of anergy in memory T cells by aaDC was not a result of the presence of CD25hi regulatory T cells and could be partially reversed by IL-2. Both T cell subsets acquired regulatory activity and inhibited primary CD4 and CD8 responses. Addition of exogenous IL-12p70 during T cell priming by aaDC prevented anergy induction in memory T cells and cytokine polarization in naïve T cells, indicating that the lack of IL-12p70 is a key feature of aaDC. Our finding that aaDC differentially regulate naïve and memory T cells is important for understanding and maximizing the therapeutic potential of aaDC.

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Uncontrolled Keywords: tolerance, cytokine deviation, hyporesponsiveness, immunotherapy
Depositing User: David Swan


Item ID: 16677
Identification Number:
ISSN: 0741-5400
Official URL:

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ORCID for David Swan: ORCID iD

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Date Deposited: 03 Oct 2023 14:44
Last Modified: 09 Oct 2023 09:45


Author: David Swan ORCID iD
Author: Amy E Anderson
Author: Bethan L Sayers
Author: Muzlifah A Haniffa
Author: Julie Diboll
Author: Xiao-Nong Wang
Author: John D Isaacs
Author: Catharien M U Hilkens


Sciences > Biomedical Sciences

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