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Sunderland Repository records the research produced by the University of Sunderland including practice-based research and theses.

Development and preliminary evaluation of the CyteCount system for the detection of antimicrobial resistance

Tattersall, Noah, Edwards, Connor, Cummerson, Emilie, Cooper, Callum, Stratford, James, Asally, Munehiro, Davenas, Corinne, Robyns, Audrey, Gray, Mark, Sagona, Antonia, Orenga, Sylvain and Karlikowska, Magdalena (2024) Development and preliminary evaluation of the CyteCount system for the detection of antimicrobial resistance. In: Letters in Applied Microbiology ECS Research Symposium 2024, 15 May 2024, University of the West of England, Bristol.

Item Type: Conference or Workshop Item (Poster)


Timely administration of appropriate antibiotics is paramount for effective sepsis management. Conventional diagnostics are time-consuming, requiring up to 48-hours, with increasing antimicrobial resistance exacerbating the risk of treatment failure. We aimed to develop a rapid antimicrobial resistance diagnostic for earlier therapeutic guidance, aiding clinicians in prescribing.

The CyteCount device was developed to study bioelectricity at a single-cell level. We used it to assess the effect of different antibiotic exposure times on viability and vitality of resistant and susceptible strains in both Gram-positive and Gram-negative bacteria. A comparison with traditional plate counting methods validated the accuracy and speed of our approach.

Results & Discussion:
CyteCount analyses detected resistance and susceptibility in <3hours for Gram-positive and Gram-negative strains. It identified more viable cells comparatively after treatment with a certain class of antibiotics, displaying a unique membrane potential response profile. Single-cell electrophysiological analyses offered additional insights.

CyteCount viability counts distinguished resistant from susceptible strains. Additional analysis enabled earlier detection of cells’ response to antibiotic stress, potentially detecting resistance in under two hours. CyteCount shows promise for early selection of appropriate antimicrobial therapy. Further work should assess its single-cell data analyses against solid-phase cytometry and study electrophysiological responses in slow-growing microorganisms.

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More Information

Depositing User: Callum Cooper


Item ID: 17667
Official URL:

Users with ORCIDS

ORCID for Callum Cooper: ORCID iD
ORCID for Mark Gray: ORCID iD

Catalogue record

Date Deposited: 29 May 2024 08:04
Last Modified: 29 May 2024 08:15


Author: Callum Cooper ORCID iD
Author: Mark Gray ORCID iD
Author: Noah Tattersall
Author: Connor Edwards
Author: Emilie Cummerson
Author: James Stratford
Author: Munehiro Asally
Author: Corinne Davenas
Author: Audrey Robyns
Author: Antonia Sagona
Author: Sylvain Orenga
Author: Magdalena Karlikowska

University Divisions

Faculty of Health Sciences and Wellbeing > School of Pharmacy and Pharmaceutical Sciences


Sciences > Biomedical Sciences

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