Quantification of micro-mixing is a fundamental issue in industrial chemical processes. Local mixing that is not “fast enough” compared with the reaction kinetics reduces the selectivity of the reaction. Micro-mixing can be characterized by chemical probe methods based on observation of a local chemical reaction that results from the competition between turbulent mixing at micro-scales and the reaction kinetics. However, real-world experimental conditions rarely comply with the grounding assumptions of this method. Starting from physical considerations, the present study aims to establish some guidelines for obtaining quantitative information from the chemical probe and for improving the accuracy of the method by an adaptive protocol. For the first aspect, an analytical approach is proposed to define the validity domain based on analysis of the turbulent time scales. For the second purpose, a novel experimental procedure is suggested that entails targeting the concentrations of the chemical species that can provide the optimal conditions for a relevant use of the chemical probe.