A novel fully-humanised 3D skin equivalent to model early melanoma invasion

Hill, David S., Robinson, Neil D. P., Caley, Matthew P., Chen, Mei, O'Toole, Edel A., Armstrong, Jane, Przyborski, Stefan and Lovat, Penny E. (2015) A novel fully-humanised 3D skin equivalent to model early melanoma invasion. Molecular Cancer Therapeutics, 14 (9). pp. 2665-2675. ISSN 1535-7163

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Abstract

Metastatic melanoma remains incurable, emphasising the acute need for improved research models to investigate the underlying biological mechanisms mediating tumour invasion and metastasis, and to develop more effective targeted therapies to improve clinical outcome. Available animal models of melanoma do not accurately reflect human disease and current in vitro human skin equivalent models incorporating melanoma cells are not fully representative of the human skin microenvironment. We have developed a robust and reproducible, fully-humanised 3D skin equivalent comprising a stratified, terminally differentiated epidermis and a dermal compartment consisting of fibroblast-generated extracellular matrix. Melanoma cells incorporated into the epidermis were able to invade through the basement membrane and into the dermis, mirroring early tumour invasion in vivo. Comparison of our novel 3D melanoma skin equivalent with melanoma in situ and metastatic melanoma indicates this model accurately recreates features of disease pathology, making it a physiologically representative model of early radial and vertical growth phase melanoma invasion.

Item Type: Article
Subjects: Sciences
Divisions: Faculty of Health Sciences and Wellbeing
Faculty of Health Sciences and Wellbeing > School of Nursing and Health Sciences
Faculty of Health Sciences and Wellbeing > School of Pharmacy and Pharmaceutical Sciences
Depositing User: Paula Normington
Date Deposited: 27 Oct 2015 15:34
Last Modified: 04 Feb 2020 09:50
URI: http://sure.sunderland.ac.uk/id/eprint/5702
ORCID for Jane Armstrong: ORCID iD orcid.org/0000-0002-5822-0597

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