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Sunderland Repository records the research produced by the University of Sunderland including practice-based research and theses.

Analyte Adsorption in LC Valve Injectors for Samples in Non-Eluting Solvents

Lough, John, Mills, M.J. and Maltas, J. (1996) Analyte Adsorption in LC Valve Injectors for Samples in Non-Eluting Solvents. Journal of Chromatography, 726 (1-2). pp. 67-75. ISSN 0021-9673

Item Type: Article


The cause of precision problems arising when using non-eluting solvents for sample solutions in order to obtain a peak compression effect in low-dispersion liquid chromatography was investigated. Quantitative evidence was obtained to support the proposition that the problems were caused by adsorption of the analyte in the injection system. This phenomenon was quite marked when aqueous buffer was used to inject into organic-aqueous mobile phases and could still be significant when there was only a few percent difference in the organic content of the mobile phase and the less eluting injection solvent. While there was evidence for adsorption onto the inner walls of the stainless-steel tubing used for sample loops, it was clear that, for this case of liquid chromatography of indomethacin, adsorption onto Vespel rotor seals was the principal contributing factor to the effect.

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Depositing User: Paula Normington


Item ID: 6411
Identification Number:
ISSN: 0021-9673
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Date Deposited: 12 Jun 2017 10:13
Last Modified: 18 Dec 2019 15:39


Author: John Lough
Author: M.J. Mills
Author: J. Maltas

University Divisions

Faculty of Health Sciences and Wellbeing
Faculty of Health Sciences and Wellbeing > School of Pharmacy and Pharmaceutical Sciences



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