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Sunderland Repository records the research produced by the University of Sunderland including practice-based research and theses.

Fabrication of photo-electrochemical biosensors for ultrasensitive screening of mono-bioactive molecules: the effect of geometrical structures and crystal surfaces

Akhtar, Naeem, Emran, Mohammed Y., Shenashen, Mohamed A., Khalifa, Hesham, Osaka, Tetsuya, Faheem, Ahmed, Homma, Takayuki, Kawarada, Hiroshi and El-Safty, Sherif (2017) Fabrication of photo-electrochemical biosensors for ultrasensitive screening of mono-bioactive molecules: the effect of geometrical structures and crystal surfaces. J. Mater. Chem. B, 5 (39). pp. 7985-7996. ISSN 2050-750X

Item Type: Article


The controlled design of biosensors based on the photo-electrochemical technique with high selectivity, sensitivity, and rapid response for monitoring of mono-bioactive molecules, particularly dopamine (DA) levels in neuronal cells is highly necessary for clinical diagnosis. Hierarchical carbon-, nitrogen-doped (CN) nickel oxide spear thistle (ST) flowers associated in single-heads (S), and symmetric and asymmetric-double heads (D and A, respectively) that are tightly connected through a micrometric dipole-like rod or trunk were fabricated by using a simple synthetic protocol. The CN-ST flower heads were decorated with dense nano-tubular like hedgehog needle skins in vertical alignments. These designated architectures are key features for creating biosensor surface electrodes for photo-electrochemical, ultrasensitive screening of mono-bioactive molecules. The exceptional electrode designs produced numerous catalytically active sites, large surface area, and high electron-transfer mobility. The active coating of carbon–nitrogen nanospheres significantly enhanced the photo-electrocatalytic activity of the prepared biosensor electrodes and prevented leakage of photocatalytic activity under long-term exposure to irradiation. Among all photo-electrochemical assays, the biosensors showed significant sensitivity and selectivity for DA in the presence of interfering molecules such as ascorbic acid (AA), uric acid (UA), adrenaline (A), and noradrenaline (NA). The photo-electrochemical property of the CN-SST-{110} crystal surface electrode showed significant sensing performance for DA in terms of unimpeded diffusion pathways, a wide concentration-detection range, and a low detection limit, even in the presence of potentially interfering molecules compared with other electrode-modified CN-DST-{111} and CN-AST-{101} crystal surfaces. Furthermore, the CN-SST photo-biosensor electrode shows potential in the selective and sensitive determination of DA in real samples, such as human serum and secreted DA from living cells. This finding indicates that the hierarchical ST biosensor may enable analytical discrimination and monitoring of DA and can be employed for clinical diagnosis application.

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Depositing User: Barry Hall


Item ID: 8519
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ISSN: 2050-750X
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ORCID for Sherif El-Safty: ORCID iD

Catalogue record

Date Deposited: 13 Dec 2017 17:07
Last Modified: 24 Nov 2020 17:00


Author: Sherif El-Safty ORCID iD
Author: Naeem Akhtar
Author: Mohammed Y. Emran
Author: Mohamed A. Shenashen
Author: Hesham Khalifa
Author: Tetsuya Osaka
Author: Ahmed Faheem
Author: Takayuki Homma
Author: Hiroshi Kawarada

University Divisions

Faculty of Health Sciences and Wellbeing
Faculty of Health Sciences and Wellbeing > School of Pharmacy and Pharmaceutical Sciences


Sciences > Chemistry

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