Close menu


Sunderland Repository records the research produced by the University of Sunderland including practice-based research and theses.

Aberrant methylation of imprinted genes is associated with negative hormone receptor status in invasive breast cancer

Barrow, Timothy, Barault, L, Ellsworth, RE, Harris, HR, Binder, AM, Valente, AL, Shriver, CD and Michels, KB (2015) Aberrant methylation of imprinted genes is associated with negative hormone receptor status in invasive breast cancer. International Journal of Cancer, 137 (3). pp. 537-547. ISSN 0020-7136

Item Type: Article


Epigenetic regulation of imprinted genes enables monoallelic expression according to parental
origin, and its disruption is implicated in many cancers and developmental disorders. The
expression of hormone receptors is significant in breast cancer as they are indicators of cancer cell
growth rate and determine response to endocrine therapies. We investigated the frequency of
aberrant events and variation in DNA methylation at nine imprinted sites in invasive breast cancer
and examined the association with estrogen and progesterone receptor status. Breast tissue and
blood from patients with invasive breast cancer (n=38) and benign breast disease (n=30) were
compared to those from healthy individuals (n=36), matched to the cancer patients by age at
diagnosis, ethnicity, BMI, menopausal status, and familial history of cancer. DNA methylation
and allele-specific expression were analyzed by pyrosequencing. Tumor-specific methylation
changes at IGF2 DMR2 were observed in 59% of cancer patients, IGF2 DMR0 in 38%, DIRAS3
DMR in 36%, GRB10 ICR in 23%, PEG3 DMR in 21%, MEST ICR in 19%, H19 ICR in 18%,
KvDMR in 8%, and SNRPN/SNURF ICR in 4%. Variation of methylation was significantly greater
in breast tissue from cancer patients than healthy individuals and benign breast disease. Aberrant
methylation of three or more sites was significantly associated with negative estrogen-alpha
(Fisher’s Exact Test, p=0.02) and progesterone-A (p=0.02) receptor status. Aberrant events and
increased variation of imprinted gene DNA methylation therefore appear to be frequent in invasive
breast cancer and are associated with negative estrogen and progesterone receptor status, without
loss of monoallelic expression.

BarrowBreastImprinting.pdf - Accepted Version

Download (1MB) | Preview

More Information

Depositing User: Timothy Barrow


Item ID: 8780
Identification Number:
ISSN: 0020-7136
Official URL:

Users with ORCIDS

ORCID for Timothy Barrow: ORCID iD

Catalogue record

Date Deposited: 26 Feb 2018 10:01
Last Modified: 18 Dec 2019 16:05


Author: Timothy Barrow ORCID iD
Author: L Barault
Author: RE Ellsworth
Author: HR Harris
Author: AM Binder
Author: AL Valente
Author: CD Shriver
Author: KB Michels

University Divisions

Faculty of Health Sciences and Wellbeing
Faculty of Health Sciences and Wellbeing > School of Nursing and Health Sciences


Sciences > Biomedical Sciences

Actions (login required)

View Item View Item