A new model for the study of high-K(+)-induced preconditioning in cultured neurones: role of N-methyl-d-aspartate and alpha7-nicotinic acetylcholine receptors.

van Rensburg, Ruan, Errington, D R, Ennaceur, Abdelkader, Lees, G, Obrenovitch, T P and Chazot, P L (2009) A new model for the study of high-K(+)-induced preconditioning in cultured neurones: role of N-methyl-d-aspartate and alpha7-nicotinic acetylcholine receptors. Journal of neuroscience methods, 177 (2). pp. 311-6. ISSN 0165-0270

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Abstract

Spreading depression (SD), whether elicited by local application of high K(+) medium to the cortical surface or by other stimuli, can increase the brain's tolerance to a subsequent, severe ischaemic insult in vivo, a phenomenon termed preconditioning. Herein, we have developed and validated a robust in vitro protocol for high-K(+)-preconditioning of cultured neurones. This new model is especially appropriate to unravel the molecular mechanisms underlying neuronal preconditioning and subsequent ischaemic tolerance. With this new, optimised preparation, preconditioning was found to be dependent upon culture day in vitro, cell density, K(+) concentration and duration of treatment. Finally, preconditioning was shown to be dependent upon N-methyl-d-aspartate (NMDA), CAM-kinase II signalling and alpha7-nicotinic (alpha7 nACh) receptor function, which is analogous to in vivo preconditioning induced by various stimuli.

Item Type: Article
Subjects: Sciences > Pharmacy and Pharmacology
Divisions: Faculty of Applied Sciences > Department of Pharmacy Health and Wellbeing
Health Sciences and Wellbeing Beacon
Units of Assessment > 03 Allied Health Professions, Dentistry, Nursing and Pharmacy (UoA)
Related URLs:
Depositing User: Abdelkader Ennaceur
Date Deposited: 19 Oct 2011 12:07
Last Modified: 24 Sep 2017 00:26
URI: http://sure.sunderland.ac.uk/id/eprint/933

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