Close menu

SURE

Sunderland Repository records the research produced by the University of Sunderland including practice-based research and theses.

Inhibition of erythrocyte cation channels by erythropoietin.

Mysina, Svetlana, Huber, Stephan M, Birka, Christina, Lang, Philipp A, Lang, Karl S, Friedrich, Björn, Risler, Teut, Wieder, Thomas and Lang, Florian (2003) Inhibition of erythrocyte cation channels by erythropoietin. Journal of the American Society of Nephrology : JASN, 14 (11). pp. 2750-7. ISSN 1046-6673

Item Type: Article

Abstract

Recombinant human erythropoietin therapy is used to counteract anemia that is the result of renal insufficiency. It stimulates the formation of peripheral blood erythrocytes by inhibiting apoptosis of erythrocyte precursor cells. Mature erythrocytes have similarly been shown to undergo apoptosis. Hyperosmotic shock and Cl(-) removal activate a Ca(2+)-permeable, ethylisopropylamiloride-inhibitable cation channel. The subsequent increase of cytosolic Ca(2+) activates a scramblase that breaks down cell membrane phosphatidylserine asymmetry, leading to annexin binding. Studied was whether channel activity and erythrocyte cell death are regulated by erythropoietin. Scatchard plot analysis disclosed low-abundance, high-affinity binding of (125)I-erythropoietin to erythrocytes. Whole cell patch clamp experiments revealed significant inhibition of the ethylisopropylamiloride-sensitive current by 1 U/ml erythropoietin. Cl(-) removal triggered annexin binding, an effect abrogated by erythropoietin (1 U/ml) but not by GM-CSF (10 ng/ml). Osmotic shock (700 mOsm) stimulated annexin binding within 24 h in the majority of the erythrocytes, an effect blunted by erythropoietin (1 U/ml) but not by GM-CSF (10 ng/ml). In the nominal absence of Ca(2+), the effect of osmotic shock was blunted and the effect of erythropoietin abolished. In hemodialysis patients, intravenous administration of erythropoietin (50 IU/kg) within 4 h decreased the number of annexin binding circulating erythrocytes. Erythropoietin binds to erythrocytes and inhibits volume-sensitive erythrocyte cation channels and thus the breakdown of phosphatidylserine asymmetry after activation of this channel. The effect could prolong the erythrocyte lifespan and may contribute to the enhancement of the erythrocyte number during erythropoietin therapy in dialysis patients.

Full text not available from this repository.

More Information

Depositing User: Svetlana Mysina

Identifiers

Item ID: 10019
ISSN: 1046-6673
URI: http://sure.sunderland.ac.uk/id/eprint/10019

Users with ORCIDS

Catalogue record

Date Deposited: 08 Oct 2018 09:43
Last Modified: 09 Oct 2018 11:06

Contributors

Author: Svetlana Mysina
Author: Stephan M Huber
Author: Christina Birka
Author: Philipp A Lang
Author: Karl S Lang
Author: Björn Friedrich
Author: Teut Risler
Author: Thomas Wieder
Author: Florian Lang

Subjects

Sciences > Biomedical Sciences

Actions (login required)

View Item (Repository Staff Only) View Item (Repository Staff Only)