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Enhanced susceptibility to erythrocyte "apoptosis" following phosphate depletion.

Birka, Christina, Lang, Philipp A, Kempe, Daniela S, Hoefling, Lena, Tanneur, Valerie, Duranton, Christophe, Nammi, Srinivas, Henke, Guido, Mysina, Svetlana, Krikov, Maxim, Huber, Stephan M, Wieder, Thomas and Lang, Florian (2004) Enhanced susceptibility to erythrocyte "apoptosis" following phosphate depletion. Pflugers Archiv : European journal of physiology, 448 (5). pp. 471-7. ISSN 0031-6768

Item Type: Article

Abstract

Among the sequelae of phosphate depletion is anaemia, due in part to a decreased life span of mature erythrocytes. Recent studies have disclosed that cellular stress leads to an increase of cytosolic Ca(2+) activity in erythrocytes thereby triggering cell shrinkage and breakdown of phosphatidylserine asymmetry of the cell membrane, both typical features of apoptosis. In the present experiments, phosphatidylserine exposure and cell size were measured by fluorescence-activated cell sorting (FACS) analysis of annexin binding and forward scatter, respectively. Erythrocytes from intact mice were compared with erythrocytes from mice exposed to a low-phosphate diet for 4 days. Annexin binding of freshly drawn erythrocytes was slightly but significantly enhanced by the low-phosphate diet. Furthermore, intracellular phosphate and ATP concentrations were significantly decreased in those erythrocytes whereas intracellular Ca(2+) activity was unaltered. Osmotic shock (exposure to 700 mOsm by addition of sucrose for 12 h), removal of Cl(-) (replaced by gluconate for 15 h) or removal of glucose (12 h) decreased cell volume and increased the number of annexin-binding erythrocytes. Interestingly, these effects were significantly larger in erythrocytes from phosphate-depleted animals. The experiments reveal a novel mechanism triggered by phosphate depletion that presumably contributes to the enhanced vulnerability and accelerated sequestration of erythrocytes and, thus, to anaemia.

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More Information

Depositing User: Svetlana Mysina

Identifiers

Item ID: 10024
ISSN: 0031-6768
URI: http://sure.sunderland.ac.uk/id/eprint/10024

Users with ORCIDS

Catalogue record

Date Deposited: 08 Oct 2018 09:14
Last Modified: 09 Oct 2018 11:01

Contributors

Author: Christina Birka
Author: Philipp A Lang
Author: Daniela S Kempe
Author: Lena Hoefling
Author: Valerie Tanneur
Author: Christophe Duranton
Author: Srinivas Nammi
Author: Guido Henke
Author: Svetlana Mysina
Author: Maxim Krikov
Author: Stephan M Huber
Author: Thomas Wieder
Author: Florian Lang

Subjects

Sciences > Biomedical Sciences

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