Close menu

SURE

Sunderland Repository records the research produced by the University of Sunderland including practice-based research and theses.

The role of ADP in endotoxin-induced equine platelet activation.

Jarvis, Gavin E., Evans, R J and Heath, M F (1996) The role of ADP in endotoxin-induced equine platelet activation. European journal of pharmacology, 315 (2). pp. 203-12. ISSN 0014-2999

Item Type: Article

Abstract

We have shown previously that endotoxin induces platelet aggregation in equine heparinised whole blood in a platelet-activating factor (PAF; 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine) dependent manner. ADP is an agonist of platelets and is present in platelet dense granules with ATP in high concentrations. An investigation was carried out to establish whether endotoxin-induced platelet activation was associated with release of platelet ATP and ADP. ADP-scavenging enzyme systems significantly inhibited endotoxin-induced aggregation. Plasma levels of adenine nucleotides were measured using a luminometric assay following incubation of heparinised equine whole blood with endotoxin (300 ng/ml). After addition of endotoxin ATP and ADP were released from the platelets and then subsequently degraded to AMP. WEB2086 (4-[3-[4-(o-chlorophenyl)-9-methyl-6H-thieno[3,2-f]-s-triazolo[4,3-a][1, 4] diazepin-2-yl]proprionyl]-morpholine) (100 nM), a competitive PAF receptor antagonist, inhibited endotoxin-induced aggregation and also inhibited the release of adenine nucleotides from the platelets. It is concluded that endotoxin-induced aggregation is dependent upon ADP released from platelet dense granules.

[img] PDF
Jarvis (1996) The role of ADP in endotoxin-induced equine platelet activation, EJP.pdf - Published Version
Restricted to Repository staff only

Download (804kB)

More Information

Depositing User: Gavin Jarvis

Identifiers

Item ID: 14430
Identification Number: https://doi.org/10.1016/S0014-2999(96)00637-1
ISSN: 0014-2999
URI: http://sure.sunderland.ac.uk/id/eprint/14430
Official URL: https://www.sciencedirect.com/science/article/abs/...

Users with ORCIDS

ORCID for Gavin E. Jarvis: ORCID iD orcid.org/0000-0003-4362-1133

Catalogue record

Date Deposited: 25 Jan 2022 12:49
Last Modified: 25 Jan 2022 13:00

Contributors

Author: Gavin E. Jarvis ORCID iD
Author: R J Evans
Author: M F Heath

University Divisions

Faculty of Health Sciences and Wellbeing > School of Medicine

Subjects

Sciences > Biomedical Sciences
Sciences > Pharmacy and Pharmacology

Actions (login required)

View Item (Repository Staff Only) View Item (Repository Staff Only)