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Bacterial community dynamics and associated genes in hydrocarbon contaminated soil during bioremediation using brewery spent grain.

Nnadi, Mabel Owupele, Bingle, Lewis and Thomas, Keith (2023) Bacterial community dynamics and associated genes in hydrocarbon contaminated soil during bioremediation using brewery spent grain. Access Microbiology, 5 (6). p. 211. ISSN 2516-8290

Item Type: Article

Abstract

Brewery spent grain (BSG) has previously been exploited in bioremediation. However, detailed knowledge of the associated bacterial community dynamics and changes in relevant metabolites and genes over time, is limited. This study investigated the bioremediation of diesel contaminated soil amended with BSG. We observed the complete degradation of three Total Petroleum Hydrocarbon (TPH C10 – C28) fractions in amended treatments as compared to one fraction in the unamended treatments. The biodegradation rate constant was higher in amended treatments (0.1021 k) than in unamended (0.059 k) and bacterial CFUs increased significantly in amended treatments. The degradation compounds observed fitted into the elucidated diesel degradation pathways and quantitative PCR results showed that the gene copy numbers of all three associated degradation genes, alkB, catA and xylE, were significantly higher in amended treatments. High-throughput sequencing of 16S rRNA gene amplicons showed that amendment with BSG enriched autochthonous hydrocarbon degraders. Also, community shifts of the genera Acinetobacter and Pseudomonas correlated with the abundance of catabolic genes and degradation compounds observed. This study showed that these two genera are present in BSG and thus, may be associated with the enhanced biodegradation observed in amended treatments. The results suggest that the combined evaluation of TPH, microbiological, metabolite and genetic analysis provides a useful wholistic approach to assessing bioremediation.

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More Information

Uncontrolled Keywords: Bacterial community profiling, alkB, catA, xylE catabolic genes, Diesel metabolites 19 Total Petroleum Hydrocarbons 20 Brewery spent grain 21 Bioremediation
Depositing User: Lewis Bingle

Identifiers

Item ID: 16141
Identification Number: https://doi.org/10.1099/acmi.0.000519.v3
ISSN: 2516-8290
URI: http://sure.sunderland.ac.uk/id/eprint/16141
Official URL: https://www.microbiologyresearch.org/content/journ...

Users with ORCIDS

ORCID for Mabel Owupele Nnadi: ORCID iD orcid.org/0000-0002-8410-5867
ORCID for Lewis Bingle: ORCID iD orcid.org/0000-0001-5168-5167
ORCID for Keith Thomas: ORCID iD orcid.org/0000-0003-1197-7522

Catalogue record

Date Deposited: 22 May 2023 11:54
Last Modified: 23 Apr 2024 08:45

Contributors

Author: Mabel Owupele Nnadi ORCID iD
Author: Lewis Bingle ORCID iD
Author: Keith Thomas ORCID iD

University Divisions

Faculty of Health Sciences and Wellbeing > School of Nursing and Health Sciences

Subjects

Sciences > Biomedical Sciences
Sciences > Chemistry
Sciences > Environment
Sciences > Health Sciences

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