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Determination of cellular glutathione: glutathione disulphide ratio in prostate cancer cells by high performance liquid chromatography with electrochemical detection

Childs, Stephen, Haroune, Nicolas, Williams, Lee and Gronow, M (2016) Determination of cellular glutathione: glutathione disulphide ratio in prostate cancer cells by high performance liquid chromatography with electrochemical detection. Journal of Chromatography A, 1437. pp. 67-73. ISSN S0021-9673

Item Type: Article

Abstract

A validated method has been developed for the simultaneous measurement of reduced and oxidized glutathione in de-proteinised cellular extracts. This has been used to compare models of malignant and non-malignant human prostate cell lines. Analysis of LNCaP and DU145 cells showed a glutathione to glutathione disulfide ratio of 8:1 and 32:1 respectively, whilst the control cell line, PZ-HPV7 displayed a ratio of 93:1. Results indicate that the more aggressive phenotype displays adaptation to increased oxidative stress via up regulation of glutathione turnover. It was also noted that in the LNCaP and DU145 cell line, glutathione was only responsible for ca. 60% and 79% respectively, of the total cellular reduced thiol; indicating the presence of other biological thiols.

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More Information

Depositing User: Paula Normington

Identifiers

Item ID: 6023
Identification Number: https://doi.org/10.1016/j.chroma.2016.01.050
ISSN: S0021-9673
URI: http://sure.sunderland.ac.uk/id/eprint/6023
Official URL: http://www.sciencedirect.com/science/article/pii/S...

Users with ORCIDS

ORCID for Stephen Childs: ORCID iD orcid.org/0000-0002-7140-7831

Catalogue record

Date Deposited: 31 Mar 2016 14:09
Last Modified: 18 Dec 2019 15:38

Contributors

Author: Stephen Childs ORCID iD
Author: Nicolas Haroune
Author: Lee Williams
Author: M Gronow

University Divisions

Faculty of Health Sciences and Wellbeing
Faculty of Health Sciences and Wellbeing > School of Pharmacy and Pharmaceutical Sciences

Subjects

Sciences

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