Close menu

SURE

Sunderland Repository records the research produced by the University of Sunderland including practice-based research and theses.

Insights into the influences of carboxymethyl-β-cyclodextrin on DNA formulations characteristics and gene transfection efficiency

Elsana, Hassan, Mysina, Svetlana, Elkordy, Emal Ali, Carr-Wilkinson, Jane and Elkordy, Amal (2018) Insights into the influences of carboxymethyl-β-cyclodextrin on DNA formulations characteristics and gene transfection efficiency. Current Drug Delivery, 15 (6). pp. 867-878. ISSN 1567 2018

Item Type: Article

Abstract

BACKGROUND

Gene therapy is an expanding field and it can treat genetic and acquired diseases.

OBJECTIVE

It was found that formulations with DNA: CM-β-CD (Carboxymethyl-beta-cyclodextrin): Pluronic-F127 1:3:3 and 1:3 DNA: CM-β-CD are the most stable formulations indicating high incorporation of DNA within CM-β -CD.

METHOD

Gel electrophoresis revealed DNA with low CM-β -CD concentration has formed a more stable complex. Samples 1:3 DNA: CM-β-CD and 1:3:3 DNA: CM-β-CD: Pluronic-127 show no DNA fragment, suggesting good condensation of DNA inside cyclodextrin cavity.

RESULTS

This was confirmed by fluorescence data where fluorescence intensity was reduced for samples DNA: CM-β-CD 1:3. Overall, the findings showed that Carboxymethyl-beta-cyclodextrin (as a novel non-viral gene vector) was able to provide condensation and protection to the DNA, with and without Pluronic-F127, at low concentration.

CONCLUSION

pDNA/CM-β-CD complex has not only shown to be able to transfect COS 7 and SHSY5Y cell lines, but it gives a higher transfection efficiency than that produced by the TransIT-LT1 commercial transfection reagent.

[img]
Preview
PDF
Author accepted version Elkordy.pdf - Accepted Version

Download (1MB) | Preview

More Information

Depositing User: Barry Hall

Identifiers

Item ID: 8974
Identification Number: https://doi.org/10.2174/1567201815666180226115503
ISSN: 1567 2018
URI: http://sure.sunderland.ac.uk/id/eprint/8974
Official URL: https://doi.org/10.2174/1567201815666180226115503

Users with ORCIDS

ORCID for Svetlana Mysina: ORCID iD orcid.org/0000-0003-3818-3255
ORCID for Amal Elkordy: ORCID iD orcid.org/0000-0002-0781-1127

Catalogue record

Date Deposited: 19 Mar 2018 11:14
Last Modified: 16 May 2022 13:00

Contributors

Author: Svetlana Mysina ORCID iD
Author: Amal Elkordy ORCID iD
Author: Hassan Elsana
Author: Emal Ali Elkordy
Author: Jane Carr-Wilkinson

University Divisions

Faculty of Health Sciences and Wellbeing
Faculty of Health Sciences and Wellbeing > School of Nursing and Health Sciences
Faculty of Health Sciences and Wellbeing > School of Pharmacy and Pharmaceutical Sciences

Subjects

Sciences > Pharmacy and Pharmacology

Actions (login required)

View Item (Repository Staff Only) View Item (Repository Staff Only)