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A new model for the study of high-K(+)-induced preconditioning in cultured neurones: role of N-methyl-d-aspartate and alpha7-nicotinic acetylcholine receptors.

van Rensburg, Ruan, Errington, D R, Ennaceur, Abdelkader, Lees, G, Obrenovitch, T P and Chazot, P L (2009) A new model for the study of high-K(+)-induced preconditioning in cultured neurones: role of N-methyl-d-aspartate and alpha7-nicotinic acetylcholine receptors. Journal of neuroscience methods, 177 (2). pp. 311-6. ISSN 0165-0270

Item Type: Article

Abstract

Spreading depression (SD), whether elicited by local application of high K(+) medium to the cortical surface or by other stimuli, can increase the brain's tolerance to a subsequent, severe ischaemic insult in vivo, a phenomenon termed preconditioning. Herein, we have developed and validated a robust in vitro protocol for high-K(+)-preconditioning of cultured neurones. This new model is especially appropriate to unravel the molecular mechanisms underlying neuronal preconditioning and subsequent ischaemic tolerance. With this new, optimised preparation, preconditioning was found to be dependent upon culture day in vitro, cell density, K(+) concentration and duration of treatment. Finally, preconditioning was shown to be dependent upon N-methyl-d-aspartate (NMDA), CAM-kinase II signalling and alpha7-nicotinic (alpha7 nACh) receptor function, which is analogous to in vivo preconditioning induced by various stimuli.

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Depositing User: Abdelkader Ennaceur

Identifiers

Item ID: 933
Identification Number: https://doi.org/10.1016/j.jneumeth.2008.10.012
ISSN: 0165-0270
URI: http://sure.sunderland.ac.uk/id/eprint/933
Official URL: http://dx.doi.org/10.1016/j.jneumeth.2008.10.012

Users with ORCIDS

ORCID for Abdelkader Ennaceur: ORCID iD orcid.org/0000-0001-6398-4383

Catalogue record

Date Deposited: 19 Oct 2011 12:07
Last Modified: 18 Dec 2019 15:32

Contributors

Author: Abdelkader Ennaceur ORCID iD
Author: Ruan van Rensburg
Author: D R Errington
Author: G Lees
Author: T P Obrenovitch
Author: P L Chazot
Author: Ruan van Rensburg
Author: D R Errington
Author: Abdelkader Ennaceur
Author: George Lees
Author: Tihomir P Obrenovitch
Author: Paul L Chazot

University Divisions

Faculty of Health Sciences and Wellbeing
Faculty of Health Sciences and Wellbeing > School of Pharmacy and Pharmaceutical Sciences

Subjects

Sciences > Pharmacy and Pharmacology

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