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Validation and quality control for ELISAs for use with human saliva samples.

Jaedicke, Katrin, Taylor, John and Preshaw, Philip (2012) Validation and quality control for ELISAs for use with human saliva samples. Journal of Immunological Methods, 377. pp. 62-65. ISSN 0022-1759

Item Type: Article

Abstract

Enzyme-linked immunosorbent assays (ELISAs) have proven to be a powerful tool for fast and reliable sample analysis, in both clinical diagnostics and in research. Most assays are now available for use with a range of different analytical fluids, including serum, plasma or urine. In recent years, saliva has drawn attention as a potentially valuable diagnostic fluid; however few ELISAs have been validated for use with saliva, or their validation is often incomplete. Saliva has a number of different physical characteristics than, for example, cell culture medium or serum and assuming an ELISA which works well with serum samples will also do so with saliva potentially could lead to erroneous data and conclusions. In this report, we provide a detailed protocol to validate any ELISA for use with saliva samples and show the results of validation procedures for 13 ELISAs for using saliva. Our findings suggest that the majority of ELISAs work reliably with saliva, even if the assay was not specifically designed for this biological fluid. However, we also report a few cases where recovery or intra-and inter-assay variations were unexpectedly high, emphasising the importance of performing a validation procedure for each assay before using it with saliva to ensure accurate and reliable data.

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More Information

Depositing User: Katrin Jaedicke

Identifiers

Item ID: 9592
Identification Number: https://doi.org/10.1016/j.jim.2012.01.010
ISSN: 0022-1759
URI: http://sure.sunderland.ac.uk/id/eprint/9592
Official URL: https://www.sciencedirect.com/science/article/pii/...

Users with ORCIDS

ORCID for Katrin Jaedicke: ORCID iD orcid.org/0000-0002-7389-5816

Catalogue record

Date Deposited: 31 May 2018 14:27
Last Modified: 18 Dec 2019 16:06

Contributors

Author: Katrin Jaedicke ORCID iD
Author: John Taylor
Author: Philip Preshaw

University Divisions

Faculty of Health Sciences and Wellbeing
Faculty of Health Sciences and Wellbeing > School of Nursing and Health Sciences

Subjects

Sciences > Biomedical Sciences
Sciences > Health Sciences
Sciences

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