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Analysis of pollutant-induced changes in mitochondrial DNA methylation

Byun, Hyang-Min and Barrow, Timothy (2015) Analysis of pollutant-induced changes in mitochondrial DNA methylation. In: Mitochondrial Medicine: Volume 2 - Manipulating Mitochondrial Function. Methods in Molecular Biology . Humana Press, pp. 271-283. ISBN 978-1-4939-2288-8

Item Type: Book Section

Abstract

There is increasing evidence that exposure to air pollutants are associated with human disease and may act through epigenetic modification of the nuclear genome, but there have been few publications describing their impact upon the mitochondrial genome. Mitochondrial DNA may be more susceptible to pollutant-induced changes via increased oxidative stress in the cell, and therefore this field of research is of growing interest. Many techniques employed to study DNA methylation of the nuclear genome are also applicable to mitochondrial epigenetic studies. In this chapter, we describe a protocol for the isolation of mitochondrial DNA from peripheral blood samples and the analysis of 5-methlycytosine content by bisulfite-pyrosequencing.

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More Information

Depositing User: Timothy Barrow

Identifiers

Item ID: 9740
Identification Number: https://doi.org/10.1007/978-1-4939-2288-8
ISSN: 1064-3745
ISBN: 978-1-4939-2288-8
URI: http://sure.sunderland.ac.uk/id/eprint/9740
Official URL: https://www.springer.com/gb/book/9781493922871

Users with ORCIDS

ORCID for Timothy Barrow: ORCID iD orcid.org/0000-0003-4551-3857

Catalogue record

Date Deposited: 26 Jul 2018 15:16
Last Modified: 18 Dec 2019 16:07

Contributors

Author: Timothy Barrow ORCID iD
Author: Hyang-Min Byun

University Divisions

Faculty of Health Sciences and Wellbeing
Faculty of Health Sciences and Wellbeing > School of Nursing and Health Sciences

Subjects

Sciences > Biomedical Sciences

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