A novel colorimetric assay for alpha-methylacyl-CoA racemase 1A (AMACR; P504S) utilizing the elimination of 2,4-dinitrophenolate

Yevglevskis, M., Lee, G.L., Nathubhai, Amit, Petrova, Y.D., James, T.D., Threadgill, M.D., Woodman, T.J. and Lloyd, M.D. (2017) A novel colorimetric assay for alpha-methylacyl-CoA racemase 1A (AMACR; P504S) utilizing the elimination of 2,4-dinitrophenolate. Chemical Communications, 53 (37). pp. 5073-5240. ISSN 1359-7345

Abstract

alpha-Methylacyl-CoA racemase (AMACR; P504S) regulates branchedchain
fatty acid degradation, activates Ibuprofen and is a recognised
cancer drug target. A novel, facile colorimetric assay was developed
based on elimination of 2,4-dinitrophenolate. The assay was used to
test 5 known inhibitors, determining IC50 and Ki values, reversibility
and characterizing irreversible inhibition.

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More Information

Depositing User: Amit Nathubhai

Identifiers

Item ID: 9786

Identification Number: https://doi.org/10.1039/c7cc00476a

ISSN: 1359-7345

URI: http://sure.sunderland.ac.uk/id/eprint/9786

Official URL: http://pubs.rsc.org/en/Journals/JournalIssues/CC#!...

Users with ORCIDS

ORCID for Amit Nathubhai: orcid.org/0000-0003-3686-2799

ORCID for M.D. Lloyd: orcid.org/0000-0002-4821-4361

Catalogue record

Date Deposited: 10 Aug 2018 07:51

Last Modified: 20 Nov 2020 15:22

Contributors

Author:	Amit Nathubhai
Author:	M.D. Lloyd
Author:	M. Yevglevskis
Author:	G.L. Lee
Author:	Y.D. Petrova
Author:	T.D. James
Author:	M.D. Threadgill
Author:	T.J. Woodman

University Divisions

Faculty of Health Sciences and Wellbeing
Faculty of Health Sciences and Wellbeing > School of Pharmacy and Pharmaceutical Sciences

Subjects

Sciences > Chemistry
Sciences

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