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Sunderland Repository records the research produced by the University of Sunderland including practice-based research and theses.

A Prototype Antibody-based Biosensor for Measurement of Salivary MMP-8 in Periodontitis using Surface Acoustic Wave Technology

Taylor, John, Jaedicke, Katrin, van de Merwe, Rachel, Bissett, Susan, Landsdowne, Nichola, Whall, Kerry, Pickering, Kimberley, Thornton, Vivienne, Lawson, Victoria, Yatsuda, Hiromi, Kogai, Takashi, Shah, Deepan, Athey, Dale and Preshaw, Philip (2019) A Prototype Antibody-based Biosensor for Measurement of Salivary MMP-8 in Periodontitis using Surface Acoustic Wave Technology. Scientific Reports, 9. ISSN 2045-2322

Item Type: Article


Periodontitis is an economically important disease which is highly prevalent worldwide. Current diagnostic approaches are time-consuming and require interpretation of multiple aspects of clinical and radiographic assessment. Chair-side monitoring of inflammatory mediators of periodontitis could provide immediate information about disease activity, which can inform patient management. We aimed to develop a novel prototype biosensor to measure salivary matrix metalloproteinase-8 (MMP-8) using specific antibodies and surface acoustic wave (SAW) technology. The analytical performance of the prototype biosensor was compared to standard enzyme-linked immunosorbent assay (ELISA) using unstimulated saliva samples obtained from patients with periodontitis before and after non-surgical treatment (N = 58), patients with gingivitis (N = 54) and periodontally healthy volunteers (N = 65). Receiver operator characteristic (ROC) analysis for distinguishing periodontitis from health revealed an almost identical performance between the sensor and ELISA assays (area under curve values (AUC): ELISA 0.93; SAW 0.89). Furthermore, both analytical approaches yielded readouts which distinguished between heath, gingivitis and periodontitis, correlated identically with clinical measures of periodontal disease and recorded similar post-treatment decreases in salivary MMP-8 in periodontitis. The assay time for our prototype device is 20 minutes. The prototype SAW biosensor is a novel and rapid method of monitoring periodontitis which delivers similar analytical performance to conventional laboratory assays.

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Depositing User: Katrin Jaedicke


Item ID: 11004
Identification Number:
ISSN: 2045-2322
Official URL:

Users with ORCIDS

ORCID for Katrin Jaedicke: ORCID iD

Catalogue record

Date Deposited: 07 Aug 2019 09:00
Last Modified: 30 Sep 2020 10:50


Author: Katrin Jaedicke ORCID iD
Author: John Taylor
Author: Rachel van de Merwe
Author: Susan Bissett
Author: Nichola Landsdowne
Author: Kerry Whall
Author: Kimberley Pickering
Author: Vivienne Thornton
Author: Victoria Lawson
Author: Hiromi Yatsuda
Author: Takashi Kogai
Author: Deepan Shah
Author: Dale Athey
Author: Philip Preshaw

University Divisions

Faculty of Health Sciences and Wellbeing
Faculty of Health Sciences and Wellbeing > School of Nursing and Health Sciences



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