Close menu


Sunderland Repository records the research produced by the University of Sunderland including practice-based research and theses.

Loss of acetylation at Lys16 and trimethylation at Lys20 of histone H4 is a common hallmark of human cancer.

Fraga, Mario F, Ballestar, Esteban, Villar-Garea, Ana, Boix-Chornet, Manuel, Espada, Jesus, Schotta, Gunnar, Bonaldi, Tiziana, Haydon, Claire, Ropero, Santiago, Petrie, Kevin, Iyer, N Gopalakrishna, Pérez-Rosado, Alberto, Calvo, Enrique, Lopez, Juan A, Cano, Amparo, Calasanz, Maria J, Colomer, Dolors, Piris, Miguel Angel, Ahn, Natalie, Imhof, Axel, Caldas, Carlos, Jenuwein, Thomas and Esteller, Manel (2005) Loss of acetylation at Lys16 and trimethylation at Lys20 of histone H4 is a common hallmark of human cancer. Nature genetics, 37 (4). pp. 391-400. ISSN 1061-4036

Item Type: Article


CpG island hypermethylation and global genomic hypomethylation are common epigenetic features of cancer cells. Less attention has been focused on histone modifications in cancer cells. We characterized post-translational modifications to histone H4 in a comprehensive panel of normal tissues, cancer cell lines and primary tumors. Using immunodetection, high-performance capillary electrophoresis and mass spectrometry, we found that cancer cells had a loss of monoacetylated and trimethylated forms of histone H4. These changes appeared early and accumulated during the tumorigenic process, as we showed in a mouse model of multistage skin carcinogenesis. The losses occurred predominantly at the acetylated Lys16 and trimethylated Lys20 residues of histone H4 and were associated with the hypomethylation of DNA repetitive sequences, a well-known characteristic of cancer cells. Our data suggest that the global loss of monoacetylation and trimethylation of histone H4 is a common hallmark of human tumor cells.

[img] HTML
ng1531 - Published Version

Download (312kB)

More Information

Depositing User: Kevin Petrie


Item ID: 12423
Identification Number:
ISSN: 1061-4036
Official URL:

Users with ORCIDS

ORCID for Kevin Petrie: ORCID iD

Catalogue record

Date Deposited: 18 Aug 2020 10:13
Last Modified: 19 Aug 2020 10:13


Author: Kevin Petrie ORCID iD
Author: Mario F Fraga
Author: Esteban Ballestar
Author: Ana Villar-Garea
Author: Manuel Boix-Chornet
Author: Jesus Espada
Author: Gunnar Schotta
Author: Tiziana Bonaldi
Author: Claire Haydon
Author: Santiago Ropero
Author: N Gopalakrishna Iyer
Author: Alberto Pérez-Rosado
Author: Enrique Calvo
Author: Juan A Lopez
Author: Amparo Cano
Author: Maria J Calasanz
Author: Dolors Colomer
Author: Miguel Angel Piris
Author: Natalie Ahn
Author: Axel Imhof
Author: Carlos Caldas
Author: Thomas Jenuwein
Author: Manel Esteller

University Divisions

Faculty of Health Sciences and Wellbeing > School of Medicine


Sciences > Biomedical Sciences
Sciences > Health Sciences

Actions (login required)

View Item View Item