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The role of ADP in endotoxin-induced equine platelet activation.

Jarvis, Gavin E., Evans, R J and Heath, M F (1996) The role of ADP in endotoxin-induced equine platelet activation. European journal of pharmacology, 315 (2). pp. 203-12. ISSN 0014-2999

Item Type: Article


We have shown previously that endotoxin induces platelet aggregation in equine heparinised whole blood in a platelet-activating factor (PAF; 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine) dependent manner. ADP is an agonist of platelets and is present in platelet dense granules with ATP in high concentrations. An investigation was carried out to establish whether endotoxin-induced platelet activation was associated with release of platelet ATP and ADP. ADP-scavenging enzyme systems significantly inhibited endotoxin-induced aggregation. Plasma levels of adenine nucleotides were measured using a luminometric assay following incubation of heparinised equine whole blood with endotoxin (300 ng/ml). After addition of endotoxin ATP and ADP were released from the platelets and then subsequently degraded to AMP. WEB2086 (4-[3-[4-(o-chlorophenyl)-9-methyl-6H-thieno[3,2-f]-s-triazolo[4,3-a][1, 4] diazepin-2-yl]proprionyl]-morpholine) (100 nM), a competitive PAF receptor antagonist, inhibited endotoxin-induced aggregation and also inhibited the release of adenine nucleotides from the platelets. It is concluded that endotoxin-induced aggregation is dependent upon ADP released from platelet dense granules.

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Depositing User: Gavin Jarvis


Item ID: 14430
Identification Number:
ISSN: 0014-2999
Official URL:

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ORCID for Gavin E. Jarvis: ORCID iD

Catalogue record

Date Deposited: 25 Jan 2022 12:49
Last Modified: 25 Jan 2022 13:00


Author: Gavin E. Jarvis ORCID iD
Author: R J Evans
Author: M F Heath

University Divisions

Faculty of Health Sciences and Wellbeing > School of Medicine


Sciences > Biomedical Sciences
Sciences > Pharmacy and Pharmacology

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