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Evaluation of RGM Medium for Isolation of Nontuberculous Mycobacteria from Respiratory Samples from Patients with Cystic Fibrosis in the United States

Plongla, Rongpong, Preece, Clair L., Perry, John D., Gilligan, Peter H. and Forbes, Betty A. (2017) Evaluation of RGM Medium for Isolation of Nontuberculous Mycobacteria from Respiratory Samples from Patients with Cystic Fibrosis in the United States. Journal of Clinical Microbiology, 55 (5). pp. 1469-1477. ISSN 0095-1137

Item Type: Article

Abstract

A novel selective agar (RGM medium) has been advocated for the isolation of rapidly growing mycobacteria from the sputa of cystic fibrosis (CF) patients. The aim of this study was to compare RGM medium to Burkholderia cepacia selective agar (BCSA) and a standard acid-fast bacillus (AFB) culture method for the isolation of nontuberculous mycobacteria (NTM) from patients with CF. The applicability of matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) for the identification of NTM isolated on RGM medium was also assessed. Respiratory samples (n = 869) were collected from 487 CF patients and inoculated directly onto RGM medium and BCSA. Cultures were incubated at 30°C and examined for up to 28 days. A subset of 212 samples (from 172 patients) was also cultured by using a mycobacterial growth indicator tube (MGIT) and on Lowenstein-Jensen medium following dual decontamination. By using a combination of all methods, 98 mycobacteria were isolated from 869 samples (11.3%). The sensitivity of RGM medium (96.9%) was significantly higher than that of BCSA (35.7%) for the isolation of mycobacteria (P < 0.0001). The sensitivity of RGM medium was also superior to that of standard AFB culture for the isolation of mycobacteria (92.2% versus 47.1%; P < 0.0001). MALDI-TOF MS was effective for the identification of mycobacteria in RGM medium. RGM medium offers a simple and highly effective tool for the isolation of NTM from patients with CF. Extended incubation of RGM medium for 28 days facilitates the isolation of slow-growing species, including members of the Mycobacterium avium complex (MAVC).

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More Information

Uncontrolled Keywords: culture, cystic fibrosis, mycobacterium, rapid grower, selective medium
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Depositing User: Clair Preece

Identifiers

Item ID: 15912
Identification Number: https://doi.org/10.1128/jcm.02423-16
ISSN: 0095-1137
URI: http://sure.sunderland.ac.uk/id/eprint/15912
Official URL: http://dx.doi.org/10.1128/jcm.02423-16

Users with ORCIDS

ORCID for Clair L. Preece: ORCID iD orcid.org/0000-0002-1290-0423

Catalogue record

Date Deposited: 27 Apr 2023 13:41
Last Modified: 30 Apr 2023 16:45

Contributors

Author: Clair L. Preece ORCID iD
Author: Rongpong Plongla
Author: John D. Perry
Author: Peter H. Gilligan
Author: Betty A. Forbes

University Divisions

Faculty of Health Sciences and Wellbeing > School of Medicine

Subjects

Sciences > Biomedical Sciences
Sciences > Health Sciences

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