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Post-transcriptional regulation of the Bacillus subtilis pst operon encoding a phosphate-specific ABC transporter.

Allenby, Nick, O'Connor, Nicola, Prágai, Zoltán, Carter, Noel M., Miethke, Marcus, Engelmann, Susanne, Hecker, Michael, Wipat, Anil, Ward, Alan C. and Harwood, Colin R. (2004) Post-transcriptional regulation of the Bacillus subtilis pst operon encoding a phosphate-specific ABC transporter. Microbiology, 150 (8). pp. 2619-2628. ISSN 1350-0872

Item Type: Article


During phosphate starvation, Bacillus subtilis regulates genes in the PhoP regulon to reduce the cell's requirement for this essential substrate and to facilitate the recovery of inorganic phosphate from organic sources such as teichoic and nucleic acids. Among the proteins that are highly induced under these conditions is PstS, the phosphate-binding lipoprotein component of a high-affinity ABC-type phosphate transporter. PstS is encoded by the first gene in the pst operon, the other four members of which encode the integral membrane and cytoplasmic components of the transporter. The transcription of the pst operon was analysed using a combination of methods, including transcriptional reporter gene technology, Northern blotting and DNA arrays. It is shown that the primary transcript of the pst operon is processed differentially to maintain higher concentrations of PstS relative to other components of the transporter. The comparative studies have revealed limitations in the use of reporter gene technology for analysing the transcription of operons in which the messenger RNA transcript is differentially processed.

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Depositing User: Nick Allenby


Item ID: 16430
Identification Number:
ISSN: 1350-0872
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ORCID for Nick Allenby: ORCID iD

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Date Deposited: 13 Sep 2023 13:26
Last Modified: 13 Sep 2023 14:03


Author: Nick Allenby ORCID iD
Author: Nicola O'Connor
Author: Zoltán Prágai
Author: Noel M. Carter
Author: Marcus Miethke
Author: Susanne Engelmann
Author: Michael Hecker
Author: Anil Wipat
Author: Alan C. Ward
Author: Colin R. Harwood

University Divisions

Faculty of Health Sciences and Wellbeing


Sciences > Biomedical Sciences

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