Existing research on college and university students shows that sexual violence is prevalent and gendered. To address this, there has been a proliferation of active bystander training programmes implemented, particularly in US colleges and universities. Evaluations of these programmes show that they have the potential to disrupt sexual violence. Emerging research on college and university students shows that transgender and non-binary students experience (in some studies higher rates of) sexual violence, misgendering, harassment, and discrimination, which impact negatively upon their mental health, academic study, safety and perceptions of this, and their everyday behaviours. Yet active bystander training programmes have been predominantly designed to disrupt sexual violence (and other harmful behaviours) experienced by cis women students given the existing research that has focused on their experiences of sexual violence. Using Interpretative Phenomenological Analysis we analysed data from semi-structured interviews with 9 university students studying in the UK, who identified as either transgender or non-binary. We found that they experienced misgendering, harassment, and sexual violence. In this article, we analyse the implications of these findings for the design and application of The Intervention Initiative, an active bystander training programme designed and recommended for use in English universities.