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Effects of methimepip and JNJ-5207852 in Wistar rats exposed to an open-field with and without object and in Balb/c mice exposed to a radial-arm maze

Abuhamdah, Rushdie M., van Rensburg, Ruan, Lethbridge, Natasha L., Ennaceur, Abdelkader and Chazot, Paul L. (2012) Effects of methimepip and JNJ-5207852 in Wistar rats exposed to an open-field with and without object and in Balb/c mice exposed to a radial-arm maze. Frontiers in Systems Neuroscience, 6. ISSN 1662-5137

Item Type: Article

Abstract

The role of the histamine H3 receptor (H3R) in anxiety is controversial, due to limitations in drug selectivity and limited validity of behavioral tests used in previous studies. In the present report, we describe two experiments. In the first one, Wistar rats were treated with an H3R agonist (methimepip), and exposed to an open-field. In the second one, Balb/c mice were treated with H3R agonist (methimepip) or antagonist (JNJ-5207852), and exposed to an open space 3D maze which is a modified version of the radial-arm maze. C57BL/6J saline treated mice were included for comparisons. When exposed to an empty open field, Wistar rats spent more time in the outer area and made very low number of brief crossings in the central area. However, when an object occupied the central area, rats crossed frequently into and spent a long time in the central area. Administration of a range of different doses of methimepip (selective H3R agonist) reduced the entries into the central area with a novel object, indicating enhanced avoidance response. In the 3D maze, both Balb/c and C57BL/6J saline-treated mice crossed frequently onto the bridges that radiate from the central platform but only C57BL/6J mice crossed onto the arms which extend the bridges. This suggests that Balb/c mice are more anxious than C57BL/6J mice. Neither methimepip nor JNJ-5207852 (selective H3R antagonist/inverse agonist) induced entry into the arms of the maze, indicative of lack of anxiolytic effects.

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Depositing User: Abdelkader Ennaceur

Identifiers

Item ID: 3180
Identification Number: https://doi.org/10.3389/fnsys.2012.00054
ISSN: 1662-5137
URI: http://sure.sunderland.ac.uk/id/eprint/3180
Official URL: http://dx.doi.org/10.3389/fnsys.2012.00054

Users with ORCIDS

ORCID for Abdelkader Ennaceur: ORCID iD orcid.org/0000-0001-6398-4383

Catalogue record

Date Deposited: 02 Nov 2012 11:30
Last Modified: 18 Dec 2019 15:34

Contributors

Author: Abdelkader Ennaceur ORCID iD
Author: Rushdie M. Abuhamdah
Author: Ruan van Rensburg
Author: Natasha L. Lethbridge
Author: Paul L. Chazot

University Divisions

Faculty of Health Sciences and Wellbeing
Faculty of Health Sciences and Wellbeing > School of Pharmacy and Pharmaceutical Sciences

Subjects

Sciences > Biomedical Sciences
Psychology > Cognitive Behaviour
Sciences > Pharmacy and Pharmacology

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