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Sunderland Repository records the research produced by the University of Sunderland including practice-based research and theses.

Chemical and Antimicrobial Profiling of Propolis from Different Regions within Libya.

Siheri, W., Zhang, T., Unekwuojo Ebiloma, G., Biddau, M., Woods, N., Yassein Hussain, M., Clements, C., Fearnley, J., Edrada Ebel, R., Paget, Timothy, Muller, S., Carter, K.C., Ferro, V.A., De Koning, H.P. and Watson, D.G. (2016) Chemical and Antimicrobial Profiling of Propolis from Different Regions within Libya. PLOS ONE, 11 (5). ISSN 1932-6203

Item Type: Article


Extracts from twelve samples of propolis collected from different regions of Libya were tested for their activity against Trypanosoma brucei, Leishmania donovani, Plasmodium falciparum, Crithidia fasciculata and Mycobacterium marinum and the cytotoxicity of the extracts was tested against mammalian cells. All the extracts were active to some degree against all of the protozoa and the mycobacterium, exhibiting a range of EC50 values between 1.65 and 53.6 μg/ml. The toxicity against mammalian cell lines was only moderate; the most active extract against the protozoan species, P2, displayed an IC50 value of 53.2 μg/ml. The extracts were profiled by using liquid chromatography coupled to high resolution mass spectrometry. The data sets were extracted using m/z Mine and the accurate masses of the features extracted were searched against the Dictionary of Natural Products (DNP). A principal component analysis (PCA) model was constructed which, in combination with hierarchical cluster analysis (HCA), divided the samples into five groups. The outlying groups had different sets of dominant compounds in the extracts, which could be characterised by their elemental composition. Orthogonal partial least squares (OPLS) analysis was used to link the activity of each extract against the different micro-organisms to particular components in the extracts.

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Depositing User: Paula Normington


Item ID: 6291
Identification Number:
ISSN: 1932-6203
Official URL:

Users with ORCIDS

ORCID for Timothy Paget: ORCID iD

Catalogue record

Date Deposited: 10 May 2016 14:49
Last Modified: 05 Feb 2020 16:26


Author: Timothy Paget ORCID iD
Author: W. Siheri
Author: T. Zhang
Author: G. Unekwuojo Ebiloma
Author: M. Biddau
Author: N. Woods
Author: M. Yassein Hussain
Author: C. Clements
Author: J. Fearnley
Author: R. Edrada Ebel
Author: S. Muller
Author: K.C. Carter
Author: V.A. Ferro
Author: H.P. De Koning
Author: D.G. Watson

University Divisions

Faculty of Health Sciences and Wellbeing
Faculty of Health Sciences and Wellbeing > School of Medicine



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