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Anti-mycobacterial assessment and characterization of 5-O-caffeoylquinic acid methyl ester and rutin from Pavetta crassipes

Odumosu, Patricia, Lough, John, Yakubu, Davis, Thomas, Keith, Williamson, Gemma and Haroune, Nicolas (2016) Anti-mycobacterial assessment and characterization of 5-O-caffeoylquinic acid methyl ester and rutin from Pavetta crassipes. Journal of Applied Pharmaceutical Science, 6 (10). 001-007. ISSN 2231-3354

Item Type: Article


Pavetta crassipes leaf (Fam. Rubiaceae) is used as part of a combination herbal remedy for the treatment of
tuberculosis (TB) and other respiratory infections in Nigerian ethno medicine. However, little scientific data is
available to support the use in ethnomedical therapy so the objective of the study was to assess the antitubercular property and to identify the bioactive components. The dried powdered leaf was sequentially
extracted with solvents to obtain hexane, dichloromethane, methanol and water extracts. Following which, the
extracts were then screened against Mycobacterium aurum, a rapidly growing saprophytic mycobacterium
species for activity. The methanol extract exhibited inhibitory activity at an MIC value of 250 µg/mL against M.
aurum and two known polyphenolic compounds were isolated as 5-O-caffeoylquinic acid methyl ester and
quercetin-3-rutinoside (rutin). Reversed phase semi-preparative HPLC, mass spectrometry and 1H and 13C
NMR techniques were utilized in isolating and characterizing the two components. The assignments of the
structures were consistent with data from the literature. The study has shown that the methanol extract has some
activity and hyphenation of LC-MS can be used for the isolation of polyphenols from the methanol fraction
without a rigorous purification process.

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Depositing User: Barry Hall


Item ID: 7241
Identification Number:
ISSN: 2231-3354
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Date Deposited: 17 May 2017 07:54
Last Modified: 05 Feb 2021 13:19


Author: Patricia Odumosu
Author: John Lough
Author: Davis Yakubu
Author: Keith Thomas
Author: Gemma Williamson
Author: Nicolas Haroune

University Divisions

Faculty of Health Sciences and Wellbeing
Faculty of Health Sciences and Wellbeing > School of Pharmacy and Pharmaceutical Sciences


Sciences > Pharmacy and Pharmacology

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