Insights into the influences of carboxymethyl-β-cyclodextrin on DNA formulations characteristics and gene transfection efficiency
Elsana, Hassan, Mysina, Svetlana, Elkordy, Emal Ali, Carr-Wilkinson, Jane and Elkordy, Amal (2018) Insights into the influences of carboxymethyl-β-cyclodextrin on DNA formulations characteristics and gene transfection efficiency. Current Drug Delivery, 15 (6). pp. 867-878. ISSN 1567 2018
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Abstract
BACKGROUND
Gene therapy is an expanding field and it can treat genetic and acquired diseases.
OBJECTIVE
It was found that formulations with DNA: CM-β-CD (Carboxymethyl-beta-cyclodextrin): Pluronic-F127 1:3:3 and 1:3 DNA: CM-β-CD are the most stable formulations indicating high incorporation of DNA within CM-β -CD.
METHOD
Gel electrophoresis revealed DNA with low CM-β -CD concentration has formed a more stable complex. Samples 1:3 DNA: CM-β-CD and 1:3:3 DNA: CM-β-CD: Pluronic-127 show no DNA fragment, suggesting good condensation of DNA inside cyclodextrin cavity.
RESULTS
This was confirmed by fluorescence data where fluorescence intensity was reduced for samples DNA: CM-β-CD 1:3. Overall, the findings showed that Carboxymethyl-beta-cyclodextrin (as a novel non-viral gene vector) was able to provide condensation and protection to the DNA, with and without Pluronic-F127, at low concentration.
CONCLUSION
pDNA/CM-β-CD complex has not only shown to be able to transfect COS 7 and SHSY5Y cell lines, but it gives a higher transfection efficiency than that produced by the TransIT-LT1 commercial transfection reagent.
Item Type: | Article |
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Subjects: | Sciences > Pharmacy and Pharmacology |
Divisions: | Faculty of Health Sciences and Wellbeing Faculty of Health Sciences and Wellbeing > School of Nursing and Health Sciences Faculty of Health Sciences and Wellbeing > School of Pharmacy and Pharmaceutical Sciences |
Depositing User: | Barry Hall |
Date Deposited: | 19 Mar 2018 11:14 |
Last Modified: | 30 Sep 2020 10:48 |
URI: | http://sure.sunderland.ac.uk/id/eprint/8974 |
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