Close menu


Sunderland Repository records the research produced by the University of Sunderland including practice-based research and theses.

Extraction, identification and biological activities of saponins in sea cucumber Pearsonothuria graeffei

Khattab, RA, Elbandy, M, Lawrence, A, Paget, Timothy, Rae-Rho, J, Binnaser, YS and Ali, I (2018) Extraction, identification and biological activities of saponins in sea cucumber Pearsonothuria graeffei. Comb Chem High Throughput Screen, 21 (3). pp. 222-231. ISSN 1386-2073

Item Type: Article


AIM AND OBJECTIVE: Secondary metabolism in marine organisms produced a diversity of biological important natural compounds which are unpresented in terrestrial
species. Sea cucumbers belong to the invertebrate Echinodermata and are famousfor their nutraceutical, medical and food values. They are known for possession
triterpenoid glycosides (saponins) with various ecological roles. The current work aimed to separate, identify and test various biological activities (anti-bacterial, antifungal, antileishmanial and anticancer properties) of
saponins produced by the holothurian Pearsonothuria graeffei from the Red Sea, Egypt.
MATERIAL AND METHOD: The structures were identified by 1D and 2D NMR (1H, 13C,TOCSY, COSY, HSQC, HMBC, and ROESY) experiments and acid hydrolysis. The crude
and purified fractions was analyzed using matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS)/MS to identify saponins and characterize their molecular structures. Partially purified fraction containing mainly compounds 1 and 2 was screened for antifungal activity against three clinical isolates of Candida albicans (Candida 580 (1), Candida 581(2) and
Candida MEO47228. Antileishmanial activity against Leishmania major and toxicity on colon cell-line were also evaluated.
RESULTS: Two lanostane type sulfated triterpene monoglycosides were isolated from the Holothurian Pearsonothuria graeffei from the Red Sea, Egypt. Holothurin A (1)and echinoside A (2) triterpene saponins were separated by reversed phase semi-preparative HPLC. LC50 values (µg/mL); calculated for the fraction
containing saponins 1 and 2 as major constituents; against Candida albicans, Leishmania major and colon cell-line were 10, 20 and 0.50, respectively.
CONCLUSION: Consequently, this study demonstrated the potential use of sea cucumber Pearsonothuria graeffei not only as appreciated functional food or
nutraceuticals but also as the source of functional ingredients for pharmaceutical products with antifungal, antileishmanial and anticancer properties.

PDF (Galley Proof)
Paget T_Combinatorial Chemistry.pdf - Accepted Version

Download (449kB) | Preview

More Information

Depositing User: Timothy Paget


Item ID: 9223
Identification Number:
ISSN: 1386-2073
Official URL:

Users with ORCIDS

ORCID for Timothy Paget: ORCID iD

Catalogue record

Date Deposited: 08 May 2018 08:46
Last Modified: 18 Dec 2019 16:06


Author: Timothy Paget ORCID iD
Author: RA Khattab
Author: M Elbandy
Author: A Lawrence
Author: J Rae-Rho
Author: YS Binnaser
Author: I Ali

University Divisions

Faculty of Health Sciences and Wellbeing
Faculty of Health Sciences and Wellbeing > School of Medicine


Sciences > Chemistry

Actions (login required)

View Item View Item