A novel colorimetric assay for alpha-methylacyl-CoA racemase 1A (AMACR; P504S) utilizing the elimination of 2,4-dinitrophenolate

Yevglevskis, M., Lee, G.L., Nathubhai, Amit, Petrova, Y.D., James, T.D., Threadgill, M.D., Woodman, T.J. and Lloyd, M.D. (2017) A novel colorimetric assay for alpha-methylacyl-CoA racemase 1A (AMACR; P504S) utilizing the elimination of 2,4-dinitrophenolate. Chemical Communications, 53 (37). pp. 5073-5240. ISSN 1359-7345

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Abstract

alpha-Methylacyl-CoA racemase (AMACR; P504S) regulates branchedchain
fatty acid degradation, activates Ibuprofen and is a recognised
cancer drug target. A novel, facile colorimetric assay was developed
based on elimination of 2,4-dinitrophenolate. The assay was used to
test 5 known inhibitors, determining IC50 and Ki values, reversibility
and characterizing irreversible inhibition.

Item Type: Article
Subjects: Sciences > Chemistry
Sciences
Divisions: Health Sciences and Wellbeing Beacon > Drug Discovery and Application Workstream
Depositing User: Amit Nathubhai
Date Deposited: 10 Aug 2018 07:51
Last Modified: 10 Aug 2018 07:51
URI: http://sure.sunderland.ac.uk/id/eprint/9786

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