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Evaluation of novel cationic gene based liposomes with cyclodextrin prepared by thin film hydration and microfluidic systems

Elsana, Hassan, Olusanya, Temidayo O. B., Carr-Wilkinson, Jane, Darby, Steven, Faheem, Ahmed and Elkordy, Amal (2019) Evaluation of novel cationic gene based liposomes with cyclodextrin prepared by thin film hydration and microfluidic systems. Scientific Reports, 9 (15120).

Item Type: Article

Abstract

In gene delivery, non-viral vectors have become the preferred carrier system for DNA delivery. They can overcome major viral issues such as immunogenicity and mutagenicity. Cationic lipid-mediated gene transfer is one of the most commonly used non-viral vectors, which have been shown to be a safe and effective carrier. However, their use in gene delivery often exhibits low transfection efficiency and stability. The aim of this study was to examine the effectiveness of novel non-viral gene delivery systems. This study has investigated the encapsulation and transfection efficiency of cationic liposomes prepared from DOTAP and carboxymethyl-β-cyclodextrin (CD). The encapsulation efficiency of the CD-lipoplex complexes were also studied with and without the addition of Pluronic-F127, using both microfluidic and thin film hydration methods. In vitro transfection efficiencies of these complexes were determined in COS7 and SH-SY5Y cell lines. Formulation stability was evaluated using liposomes size, zeta potential and polydispersity index. In addition, the external morphology was studied using transmission electron microcopy (TEM). Results revealed that formulations produced by microfluidic method had smaller, more uniform and homogenious size and zeta-potential as well as higher encapsulation efficiency when compared with liposomes manufactured by thin film hydration method. Overall, the results of this study show that carboxymethyl-β-cyclodextrin increased lipoplexes’ encapsulation efficiency using both NanoAssemblr and rotary evaporator manufacturing processes. However, this increase was reduced slightly following the addition of Pluronic-F127. The addition of carboxymethyl-β-cyclodextrin to cationic liposomes resulted in an increase in transfection efficiency in mammalian cell lines. However, this increase appeared to be cell line specific, COS7 showed higher transfection efficiency compared to SH-SY5Y.

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More Information

Depositing User: Amal Elkordy

Identifiers

Item ID: 11266
Identification Number: https://doi.org/10.1038/s41598-019-51065-4
URI: http://sure.sunderland.ac.uk/id/eprint/11266
Official URL: https://www.nature.com/articles/s41598-019-51065-4

Users with ORCIDS

ORCID for Steven Darby: ORCID iD orcid.org/0000-0002-7226-8634
ORCID for Amal Elkordy: ORCID iD orcid.org/0000-0002-0781-1127

Catalogue record

Date Deposited: 25 Oct 2019 08:38
Last Modified: 30 Sep 2020 11:18

Contributors

Author: Steven Darby ORCID iD
Author: Amal Elkordy ORCID iD
Author: Hassan Elsana
Author: Temidayo O. B. Olusanya
Author: Jane Carr-Wilkinson
Author: Ahmed Faheem

University Divisions

Faculty of Health Sciences and Wellbeing
Faculty of Health Sciences and Wellbeing > School of Nursing and Health Sciences
Faculty of Health Sciences and Wellbeing > School of Pharmacy and Pharmaceutical Sciences

Subjects

Sciences > Pharmacy and Pharmacology

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