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Transit amplification in the amniote cerebellum evolved via a heterochronic shift in NeuroD1 expression

Butts, Thomas, Hanzel, Michalina and Wingate, Richard J. T. (2014) Transit amplification in the amniote cerebellum evolved via a heterochronic shift in NeuroD1 expression. Development, 141 (14). pp. 2791-2795. ISSN 0950-1991

Item Type: Article

Abstract

The cerebellum has evolved elaborate foliation in the amniote lineage as a consequence of extensive Atoh1-mediated transit amplification in an external germinal layer (EGL) comprising granule cell precursors. To explore the evolutionary origin of this layer, we have examined the molecular geography of cerebellar development throughout the life cycle of Xenopus laevis. At metamorphic stages Xenopus displays a superficial granule cell layer that is not proliferative and expresses both Atoh1 and NeuroD1, a marker of postmitotic cerebellar granule cells. Premature misexpression of NeuroD1 in chick partially recapitulates the amphibian condition by suppressing transit amplification. However, unlike in the amphibian, granule cells fail to enter the EGL. Furthermore, misexpression of NeuroD1 once the EGL is established both triggers radial migration and downregulates Atoh1. These results show that the evolution of transit amplification in the EGL required adaptation of NeuroD1, both in the timing of its expression and in its regulatory function, with respect to Atoh1.

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More Information

Depositing User: Leah Maughan

Identifiers

Item ID: 14345
Identification Number: https://doi.org/10.1242/dev.101758
ISSN: 0950-1991
URI: http://sure.sunderland.ac.uk/id/eprint/14345
Official URL: http://dx.doi.org/10.1242/dev.101758

Users with ORCIDS

ORCID for Thomas Butts: ORCID iD orcid.org/0000-0002-1781-5287

Catalogue record

Date Deposited: 19 Jan 2022 10:57
Last Modified: 19 Jan 2022 10:57

Contributors

Author: Thomas Butts ORCID iD
Author: Michalina Hanzel
Author: Richard J. T. Wingate

University Divisions

Faculty of Health Sciences and Wellbeing > School of Medicine

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