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Evaluation of a Novel Chromogenic Medium for the Detection of Pseudomonas aeruginosa in Respiratory Samples from Patients with Cystic Fibrosis

Truong, Thang Viet, Twist, Alexander, Zaytsev, Andrey, Marrs, Emma C. L., Perry, Audrey, Turnbull, Graeme, Orenga, Sylvain, Stanforth, Stephen P. and Perry, John D. (2022) Evaluation of a Novel Chromogenic Medium for the Detection of Pseudomonas aeruginosa in Respiratory Samples from Patients with Cystic Fibrosis. MICROORGANISMS, 10 (5). ISSN 2076-2607

Item Type: Article


Pseudomonas aeruginosa is a dominant cause of respiratory infection in individuals with cystic fibrosis (CF), leading to significant morbidity and mortality. Detection of P. aeruginosa is conducted by culture of respiratory samples but this process may occasionally be compromised due to overgrowth by other bacteria and fungi. We aimed to evaluate a novel chromogenic medium, Pseudomonas aeruginosa chromogenic agar (PACA), for culture of P. aeruginosa from respiratory samples, from patients with CF. A total of 198 respiratory samples were cultured onto PACA and three other media: CHROMID® P. aeruginosa, CHROMagar™ Pseudomonas and MacConkey agar. P. aeruginosa was recovered from 66 samples (33%), using a combination of all media. After 72 h incubation, the sensitivity of the four chromogenic media was as follows: 91% for PACA and CHROMagar™ Pseudomonas, 85% for CHROMID® P. aeruginosa and 83% for MacConkey agar. For the three chromogenic media, the positive predictive value after 72 h was as follows: 95% for PACA, 56% for CHROMagar™ Pseudomonas and 86% for CHROMID® P. aeruginosa. PACA proved to be a highly effective culture medium for the isolation and specific detection of P. aeruginosa from respiratory samples.
Keywords: Pseudomonas aeruginosa; cystic fibrosis; culture media; chromogenic; aminopeptidase

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Depositing User: Thang Truong


Item ID: 16299
Identification Number:
ISSN: 2076-2607
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Users with ORCIDS

ORCID for Thang Viet Truong: ORCID iD

Catalogue record

Date Deposited: 08 Aug 2023 15:42
Last Modified: 08 Aug 2023 15:42


Author: Thang Viet Truong ORCID iD
Author: Alexander Twist
Author: Andrey Zaytsev
Author: Emma C. L. Marrs
Author: Audrey Perry
Author: Graeme Turnbull
Author: Sylvain Orenga
Author: Stephen P. Stanforth
Author: John D. Perry

University Divisions

Faculty of Health Sciences and Wellbeing > School of Pharmacy and Pharmaceutical Sciences


Sciences > Chemistry

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